[1]邓红梅,马超,王颖.黄瓜幼苗子叶原生质体的分离纯化及融合条件优化研究[J].北方园艺,2013,37(11):6-9.
　DENG Hong-mei,MA Chao,WANG Ying.Study on the Optimal of Separation,Purification and Fusion Conditions of Cucumber Seedlings Cotyledons Protoplast[J].Northern Horticulture,2013,37(11):6-9.
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黄瓜幼苗子叶原生质体的分离纯化及融合条件优化研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年11期 页码: 6-9 栏目: 出版日期: 2013-06-15
Title:
Study on the Optimal of Separation,Purification and Fusion Conditions of Cucumber Seedlings Cotyledons Protoplast
文章编号:
1001-0009(2013)11-0006-04
作者:
邓红梅; 马超; 王颖
广东石油化工学院 化学与生命科学学院，广东 茂名 525000
Author(s):
DENG Hong-mei; MA Chao; WANG Ying
College of Chemistry and Life Sciences,Guangdong University of Petrochemical Industry,Maoming,Guangdong 525000
关键词:
黄瓜子叶; 原生质体; 分离; 纯化; 融合
Keywords:
cucumber cotyledons; protoplast; separation; purification; fusion
分类号:
S 642.2
文献标志码:
A
摘要:
以黄瓜幼苗子叶为试材，采用正交实验，探讨了不同酶浓度、渗透剂浓度、酶解时间对原生质体产量和活性的影响，并研究了不同蔗糖浓度对原生质体纯化和不同PEG浓度对原生质体融合率的影响。结果表明：黄瓜幼苗子叶在含1.0%纤维素酶、0.8%果胶酶和0.3 mol/L甘露醇的混合酶液中酶解3 h，获得具有活力的原生质体最高，为7.2×10.6个/g，原生质体活力为83.72%。蔗糖浓度为20%时纯化效果最好，得到有活力原生质体7.1×10.6个/g；PEG浓度为40%时融合率最高，融合率为32%。
Abstract:
Taking cucumber seedlings cotyledons as materials,the effect of different enzyme concentration,osmotic agent concentration,enzyme solution to time on protoplast yield and activity with orthogonal experiment were studied;and different concentration of sucrose protoplast purification and different PEG concentration on protoplast fusion rate were discussed.The results showed that cucumber seedlings in cotyledons containing 1.0% cellulose enzyme,0.8% pectinase and 0.3 mol/L of mannitol in liquid enzymes mix enzyme solution 3 hours,would obtain the highest vibrant protoplast,about 7.2×10.6pieces/g,protoplast vigor for 83.72%.When sucrose density was 20% the purified effect was the best,it could get vigorous protoplast yield 7.1×10.6pieces/g；PEG concentration of 40% had the highest rate in the fusion,fusion rate of 32%.

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备注/Memo

第一作者简介：邓红梅（1965-），女，本科，副教授，现主要从事生物技术研究工作。E-mail：dhm005@126.com.

收稿日期：2013-03-06

更新日期/Last Update: 2014-08-16