LIANG Mei-xia.Study on Shoot Tips Culture and Rapid Propagation of Forsythia suspensa[J].Northern Horticulture,2012,36(18):139-141.
连翘茎尖培养与快速繁殖研究
- Title:
- Study on Shoot Tips Culture and Rapid Propagation of Forsythia suspensa
- 文章编号:
- 1001-0009（2012）18-0139-03
- Keywords:
- Forsythia suspensa; shoot tips; rapid propagation
- 分类号:
- S 567.23+9
- 文献标志码:
- B
- 摘要:
- 以连翘茎尖为外植体，接种到不同植物生长调节剂配比的培养基上，观察连翘茎尖在不同培养基上的生长情况。结果表明：连翘茎尖初代培养的最佳培养基是MS+6-BA 2.0 mg/L+NAA 0.2 mg/L。增殖培养的最佳培养基是MS+6-BA 1.0 mg/L+NAA 0.1 mg/L，增殖系数达5.0；生根培养基是1/2 MS+NAA 0.2 mg/L，生根率达93.3%，平均每株苗生根数6.13条；连翘茎尖从接种到生根1个周期为45 d。
- Abstract:
- The shoot tips of Forsythia suspensa were used as explants and inoculated on different medium with different concentration combination for observing their growth. The results showed that the optimal medium for growth of Forsythia suspensa shoot tips was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L at the beginning of the culture.The optimal medium for inducing adventitious buds was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L. The proliferation coefficient was highest to 5.0. The rooting rate reached to 93.3% in medium 1/2 MS+NAA 0.2 mg/L and the number of the root was 6.13 per regeneration plant at average. It took 45 days for the rooting of Forsythia suspensa Vahl from the shoot tip inoculated on the medium.
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备注/Memo
作者简介：梁美霞（1970-），女，山东莱阳人，博士，副教授，现主要从事园艺植物组织培养和果树生物技术育种研究工作。E-mail：meixialiang@yahoo.com.cn。