ZHANG Tie-gang,NING Xue-fei,WANG Xian-lei,et al.Establishment of Regeneration System for Cucumis melo L.cv.‘Queen’ of Xinjiang Melon[J].Northern Horticulture,2012,36(15):122-125.
新疆甜瓜“皇后”再生体系的建立
- Title:
- Establishment of Regeneration System for Cucumis melo L.cv.‘Queen’ of Xinjiang Melon
- 文章编号:
- 1001-0009(2012)15-0122-04
- 分类号:
- S 652
- 文献标志码:
- A
- 摘要:
- 以厚皮甜瓜“皇后”为试材，研究了6-BA及其与NAA、GA3组合对植株离体再生的影响，以期建立以子叶为外植体的快速高效再生体系。结果表明：甜瓜子叶用MSB+1.0 mg/L 6-BA诱导分化培养基，不定芽诱导率可达86%以上；培养基中添加NAA不利于“皇后”子叶不定芽的产生；产生的不定芽丛或单芽转至MSB+0.1 mg/L 6-BA+0.1 mg/L GA3培养基，不定芽均可伸长，且生长状况良好；再生苗转至不添加任何激素的MS培养基生根，生根率达99%。
- Abstract:
- An efficient and rapid regeneration system of thick-skinned muskmelon had been set up using cultivars ‘Queen’ cultured in the mediums with different hormone combination of 6-BA and NAA，GA3 or only 6-BA.The results showed that the MSB medium containing 1 mg/L 6-BA was the most suitable for inducing adventitious buds with a average rate of 86%.The medium containing NAA was not favorable for the induction of adventitious bus.Adventitious buds were able to enlongate on MSB medium with 0.1 mg/L 6-BA and 0.1 mg/L GA3.Regenerated shoots were rooted on MS medium without plant grows regulators with 99% success rate.
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备注/Memo
第一作者简介：张铁刚（1985-），男，硕士，现主要从事植物生物学研究工作。E-mail：zhangtiegang02@163.com.