[1]李佳,袁素霞,刘春,等.影响百合游离小孢子活力与发育因素分析[J].北方园艺,2012,36(03):59-63.
　LI Jia,YUAN Su-xia,LIU Chun,et al.Factors Affecting Viability and Development of Isolated Microspore in Lilium[J].Northern Horticulture,2012,36(03):59-63.

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影响百合游离小孢子活力与发育因素分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷期数: 2012年03期页码: 59-63 栏目: 出版日期: 2012-03-16

Title:: Factors Affecting Viability and Development of Isolated Microspore in Lilium

文章编号:: 1001-0009（2012）03-0059-05

作者:: 李佳¹; 袁素霞¹; 刘春¹; 田梅²; 于娜丽¹; 3; 1.中国农业科学院蔬菜花卉研究所，北京 100081；
2.银川市兴庆区农牧局，宁夏银川 750001；
3.南京林业大学风景园林学院，江苏南京210037

Author(s):: LI Jia¹; YUAN Su-xia¹; LIU Chun¹; TIAN Mei²; YU Na-li¹; 3; 1.Institute of Vegetables and Flowers,Chinese Academy of Agricultural Science,Beijing 100081；
2.Xingqing District,Yinchuan Agriculture and Animal Husbandry,Ningxia,Yinchuan 750001；
3.College of Landscape Gardens，Nanjing Forestry University，Nanjing，Jiangsu 210037

关键词:: 百合; 小孢子培养; 小孢子活力; 花粉母细胞; 减数分裂

Keywords:: Lilium; microspore culture; viability of microspore; pollen mother cells; meiosis

分类号:: S 682.2+9

文献标志码:: A

摘要:: 为建立百合游离小孢子培养体系，该试验以1个野生种和5个杂种系共6份百合为试材，对小孢子发育阶段与花蕾及花药外部形态的关系、游离小孢子培养的培养基类型及基因型对小孢子发育与活力的影响进行了研究。结果表明：小孢子处于单核期的花蕾长度因不同基因型而异。处于单核期的花药颜色均为浅黄色，花粉颜色为淡黄色，可作为采样适宜期表型指标。与NLN和PG-96M培养基相比，百合不同基因型的游离小孢子在FHG培养基中能保持较高的活力且能诱导小孢子分裂。因此，FHG培养基可作为百合游离小孢子培养的首选基本培养基。不同基因型花粉母细胞减数分裂的异常率与杂合度、小孢子的发育及活力紧密相关，岷江百合（Lilium regale Wils.）及‘Tresor’在培养期间花粉母细胞减数分裂异常率低、小孢子活力较高且活力下降速度较慢；杂合度高的品种‘Robina’则异常率较高、活力下降较快。因而染色体异常率低的野生品种和亚洲百合品种更适合作为百合游离小孢子培养的首选材料。 

Abstract:: To establish the culture system of isolated microspore in Lilium,the relationship between phenotypic traits of flower bud and the meiotic process,the effects of media and genotype on vitality and development of microspores were studied in one wild species and five cultivers of Lilium.The results showed that the buds size containing uninucleate stage microspores varied with the different genotypes.While microspores were at early and middle uninucleate stage,the anther color was light yellow and the pollen color was pale yellow.Compared with NLN and PG-96M medium,in FHG medium microspores maintained higher vitality rate in all genotypes,and microspores with two symmetrical nuclei have been observed in ‘Ceb Dazzle’.In addition,the frequency of abnormal meiotic of pollen mother cell was closely related with heterozygosity and microspore viability.For example,Lilium regale Wils and ‘Tresor’ with low abnormal meiotic rate could make viability of microspores high and declining slowly in microspore culture,whereas the variety ‘Robina’ with highly heterozygosity had higher abnormal meiotic rate and fast declined in microspore vitality.

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备注/Memo

第一作者简介：李佳（1984-），女，硕士，研究方向为观赏园艺生物技术。E-mail：jjcqq0420@gmail.com。

责任作者：袁素霞（1979-），女，博士，助理研究员，研究方向为园艺作物育种及生物技术。E-mail：ysxia8@126.com。

基金项目：国家社会公益研究专项资助项目（2005DIB3J022）；国家“863”计划资助项目（2006AA100109）；“十一五”国家科技支撑计划资助项目（2006BAD01A18）；北京市花卉重点资助项目(YLHH2006001、YLHH201000101)。

收稿日期：2011-11-17

更新日期/Last Update: 2014-08-25