GUO Lei,KAN Huan,FAN Fang-yu,et al.Study on Extraction and Purification Process of Polysaccharides in Neosinocalamus affinis (Rendle) Keng f. Leaves[J].Northern Horticulture,2012,36(02):167-171.
慈竹竹叶多糖的提取及纯化工艺
- Title:
- Study on Extraction and Purification Process of Polysaccharides in Neosinocalamus affinis (Rendle) Keng f. Leaves
- 文章编号:
- 1001-0009(2012)02-0167-05
- Keywords:
- Neosinocalamus affinis (Rendle) Keng f.; polysaccharides; response surface methodology; decoloration; purification
- 分类号:
- S 7955
- 文献标志码:
- A
- 摘要:
- 以水为溶剂，以提取时间、温度和料液比3个因素作为研究对象，以多糖提取量为评价指标，在单因素的基础上，通过三因素三水平的Box-Behnken响应面分析法研究了优化多糖的提取工艺条件。结果表明：最优提取条件为提取时间143 h、温度768℃、料液比1∶803，在此条件下，多糖得率可达到411 mg/g。提取液经一系列纯化工艺后，得到纯度为6132%多糖。
- Abstract:
- Polysaccharides in Neosinocalamus affinis (Rendle) Keng f. levels was extracted by water to produce.Single-factor method was adopted to investigate the effects of extract-time,temperature and solid-liquid ratio on degree of extraction content of polysaccharides.Subsequently,a central composite design(CCD) involving 17 experiments of three variables(i.e.,extract-time,temperature and solid-liquid ratio) at three levels combined with response surface methodology was employed to attain the highest extraction content.The results showed that the optimal extract-time,temperature,solid-liquid ratio were 143 h,768℃,1∶803,respectively.On this condition,the content of polysaccharides was 411 mg/g.By the method of a series of purification process,the purity of polysaccharides was 6132%.
参考文献/References:
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备注/Memo
第一作者简介：郭磊(1981-)，男，河南卢氏人，硕士，讲师，研究方向为食品资源开发及利用。E-mail：guoleigift.student@sina.com。