SU Jiang,CEN Zhong-yong,LU Yi-cong.Effects of Different Season and Explants on the Forming of Bud Initiation of Dianthus caryophyllus[J].Northern Horticulture,2012,36(02):129-131.
不同时期及部位的外植体对香石竹芽形成的影响
- Title:
- Effects of Different Season and Explants on the Forming of Bud Initiation of Dianthus caryophyllus
- 文章编号:
- 1001-0009(2012)02-0129-03
- Keywords:
- Dianthus caryophyllus; explants; bud initiation
- 分类号:
- S 6815036
- 文献标志码:
- A
- 摘要:
- 在春、冬季取香石竹的顶芽、茎段芽进行离体培养,研究不同采集季节及部位的外植体对香石竹芽形成的影响。结果表明:在春季取顶芽做外植体采用70%酒精30 s+01%升汞 6 min消毒处理可获得较好的试管苗。70%酒精30 s+01%升汞 6 min较适宜于外植体消毒,在春季取材,顶芽分化优于茎段芽,在冬季取材,茎段芽分化优于顶芽。
- Abstract:
- Terminal buds and stem buds of Dianthus caryophyllus were used as materials for tissue culture in spring and winter,the effects of different season and explants on the forming of bud initiation of Dianthus caryophyllus were studied.The results showed that terminal buds used as explants in spring,which was disinfected by 70% alcohol 30 s with 01% HgCl-26 min could obtain best plantlets.The best disinfection effect for explants was 70% alcohol 30 s with 01% HgCl-26 min,terminal bud initiation was superior to stem buds initiation in spring,however,stem buds initiation was superior to terminal bud initiation in winter.
参考文献/References:
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备注/Memo
第一作者简介:苏江(1980-),女,壮族,广西宁明人,硕士,讲师,现主要从事植物组织培养及相关的生物技术研究工作。E-mail:supersujiang@sina.com。