|Table of Contents|

Application of Tetra-primer ARMS PCR in Development of Molecular Markers for Powdery Mildew Resistance of Bitter Melon

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年05
Page:
39-46
Research Field:
Publishing date:

Info

Title:
Application of Tetra-primer ARMS PCR in Development of Molecular Markers for Powdery Mildew Resistance of Bitter Melon
Author(s):
WANG Qixu1; 2; FENG Chengcheng2; 3; LIANG Jiazuo2; 3; CHEN Xiaofeng2; 3; HUANG Yuhui2; 3; HUANG Rukui2; 3
(1.Agricultural College，Guangxi University，Nanning，Guangxi 530005；2.Guangxi Crops Genetic and Improvement Biotechnology Lab，Nanning，Guangxi 530005；3.Vegetable Research Institute，Guangxi Academy of Agricultural Sciences，Nanning，Guangxi 530005)
Keywords:
bitter melon; primer design; tetra-primer ARMS PCR; system optimization; screening
PACS:
-
DOI:
10.11937/bfyy.20173923
Abstract:
Parental lines with high bitter gourd resistance and susceptible powdery mildew and its F2 generation were used as test materials.The tetra-primer ARMS PCR molecular marker primers were designed according to the SNP mutation sites obtained by double-sequence sequencing.Orthogonal design L16(44) method was used to optimize the system of template DNA，dNTPs，Mg2 +，Taq  DNA polymerase and internal and outer-primer concentration ratio，and then the optimized system was used to screen the primers.The tetra-primer ARMS PCR molecular marker development were suitable for bitter melon and primer screening system were studied in order to provide reference for molecular marker-assisted selection of bitter melon-resistant powdery mildew.The results showed that the optimized the tetra-prime ARMS PCR system could be correctly typed in parents and their F2 generation and was suitable for the screening of the tetra-prime ARMS PCR molecular marker which was against powdery mildew.

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Last Update: 2018-03-15