|Table of Contents|

Salinity Tolerance in Transgenic Kale Expressing the Betaine  Aldehyde Dehydrogenase Gene

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年05
Page:
33-38
Research Field:
Publishing date:

Info

Title:
Salinity Tolerance in Transgenic Kale Expressing the Betaine  Aldehyde Dehydrogenase Gene
Author(s):
LI Chunzhi1; PAN Caixia2; LIU Hongwei2; LIU Bing2; LUO Xiaoming2; LI Dahong3
(1.Zhumadian Landscaping Architecture and Research Institute，Zhumadian，Henan 463000；2.Green Department of Zhumadian，Zhumadian，Henan 463000；3.College of Biological and Food Engineering，Huanghuai University，Zhumadian，Henan 463000)
Keywords:
kale; BADH gene; transgenic plant; salt tolerance
PACS:
-
DOI:
10.11937/bfyy.20172895
Abstract:
Kale (Brassica oleracea L.var.accephala DC.) is one of the most popular and widely ornamental plants in the world.In this study，BADH (betaine aldehyde dehydrogenase) gene derived from spinach was transformed into the genome of kale mediated by Agrobacterium tumefaciens.Seven transgenic plants were obtained，and PCR，Southern blot and RT-qPCR analyses were conducted.The results showed that the BADH gene was integrated into kale genome，transcribed and expressed in the transgenic plants.The BADH enzymes activity of transgenic plants were tested after treating with NaCl stress.It showed that the average values of the activity of BADH enzymes in transgenic plants were higher than that of WT plants，and there was a significant difference between transgenic and WT.Furthermore，MDA content and the average value of relative electronic conductivity of transgenic plants were significantly lower than that of WT plants，which indicated that the protection ability of membrane penetration was enhanced when BADH activity increasing and the stress resistance of the transgenic plants was improved by introduction BADH gene into kale.At the same time，the transgenic kale plants were vigorously growing under salt stress.The average main root length，fresh weight，survival rate and chlorophyll content of transgenic plants were significantly higher than that of WT plants，while dry/fresh weight of transgenic plants was obviously lower than that of WT plants.Those results showed that the traits of salt tolerance of transgenic kale plants were improved after transforming with BADH gene，and these transgenic plants would be used as fundamental germplasm for stress-tolerant breeding of kale.

References:

 

[1]顾卫红，郑洪建，张燕，等.观赏型羽衣甘蓝新品系的选育及其主要遗传性状的传递规律初探[J].上海交通大学学报(农业科学版)，2002，20(2):129-132.

[2]YILMAZ J L，BLOW L.Enhanced stress tolerance in Escherichia coli and Nicotiana tabacum expressing a betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein[J].Biotechnology Progress，2002，18(6):1176.

[3]McCUE K F，HANSON A D.Salt-inducible betaine aldehyde dehydrogenase from sugar beet:cDNA cloning and expression[J].Plant Mol Biol，1992，18:1-11.

[4]肖岗.山菠菜甜菜碱醛脱氢酶基因研究[J].科学通报，1995，40:741-745.

[5]ISHITANI M，NAKAMURA M，HAN S Y，et al.Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress[J].Plant Mol Biol，1995，27:307-315.

[6]LI G，WU H，SUN Y，et al.Betaine Aldehyde Dehydrogenase (BADH) expression and betaine production in sugarbeet cultivars with sifferent tolerances to drought stress[J].Sugar Tech，2016，18(4):420-423.

[7]WOOD A J，SANEOKA H，RHODES D，et al.Betaine aldehyde dehydrogenase in sorghum[J].Plant Physiol，1996，110:1301-1308.

[8]郭岩，张莉.甜菜碱醛脱氢酶基因在水稻中的表达及转基因植株的耐盐性[J].中国科学(生命科学)，1997，27(2):151-155.

[9]张艳敏，郭北海，蒋春志，等.转甜菜碱醛脱氢酶(BADH)基因小麦的耐盐耐旱性[J].华北农学报，2003，18(1):29-32.

[10]ZHENG C，WANG P，NAN W U，et al.Study on drought and salt resistance of transgenic maize inbred line containing BADH[J].Journal of Jilin Agricultural University，2016，38(3):266-273.

[11]ALI A，IQBAL M S，NASIR I A.Salt tolerance of potato:Genetically engineered with atriplex canescens BADH gene driven by 3 copies of CAMV35s promoter[J].J Biotechnol Biomater，2017(7):1.

[12]高航，高玉亮，李葵花.羽衣甘蓝下胚轴农杆菌介导遗传转化体系的优化[J].生物技术通报，2015(6):111-115.

[13]吴建祥，李桂新.分子生物学实验(高等院校实验实训系列规划教材)[M].杭州:浙江大学出版社，2014.

[14]LIU F，GUO Y，GU D，et al.Salt tolerance of transgenic plants with BADH cDNA[J].Acta Genetica Sinica，1997，24(1):54-58.

[15]张志良，瞿伟菁.植物生理学实验指导[M].3版.北京:高等教育出版社，2003.

[16]LIU J，ZHOU S F，CHEN H，et al.Agrobacterium mediated transformation of BADH to the 〖STBX〗AtNHX1 〖STBZ〗transgenic tomato (Lycopersicum esculentum L.‘Moneymaker’)[J].Scientia Agricultura Sinica，2005，6(7):1064.

[17]ZHU X，CHEN W，LI X.Molecular cloning of Betaine Aldehyde Dehydrogenase (BADH) gene from harvested papaya(Carica papaya) fruit and its response to temperature stress[J].Research Journal of Biotechnology，2015，10(7):33-42.

[18]LI G，WU H，SUN Y，et al.Betaine Aldehyde Dehydrogenase (BADH) expression and betaine production in sugarbeet cultivars with different tolerances to drought stress[J].Sugar Tech，2016，18(4):420-423.

[19]余爱丽，姚伟，周会，等.高粱BADH1基因的克隆与序列分析[J].农业生物技术学报，2005，13(2):256-257.

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Last Update: 2018-03-15