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Cultivation of Coprinus comatus Using the Fermented Chinese Medicine  Residue From Yinqiao Detoxication Pills Production Waste and  Analysis of the Active Pharmaceutical Ingredient(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年02
Page:
145-149
Research Field:
Publishing date:

Info

Title:
Cultivation of Coprinus comatus Using the Fermented Chinese Medicine  Residue From Yinqiao Detoxication Pills Production Waste and  Analysis of the Active Pharmaceutical Ingredient
Author(s):
HAN Wenqing1; WANG Jianguo2; HE Yuanyuan2; YANG Li2; YANG Nana3; ZHANG Mingdou4
(1.School of Food and Drug，Baotou Light Industry Vocational Technical College，Baotou,Inner Mongolia 014035；2.Baotou Chinese Traditional Medicine Co.Ltd.，Inner Mongolia,Baotou 014040；3.Kunqu Buerhan Township Health Centers in Baotou，Baotou,Inner Mongolia 014040；4.No.2 Salaqi Senior High School，Baotou,Inner Mongolia 014100)
Keywords:
Yingqiao residue; stack fermentation; cultivation; Coprinus comatus; chlorogenic acid; arctiin
PACS:
-
DOI:
10.11937/bfyy.201702035
Abstract:
Coprinus comatus was cultivated by using the fermented Chinese medicine residue from Yinqiao detoxication pills production waste to recycle it.Corncobs were used as the main materials added with cow dung and urea to design the stack with C/N as 40∶1.Four designs A,B,C,D were adopted to build the stacks for controlling with Design A：corncobs 200 kg，cow dung 100 kg，urea 3.5 kg，accessories 6%(lime 2%,gypsum 2%,calcium superphosphate 2%).Design B：corncobs 100 kg，Yingqiao residue 50 kg，cow dung 50 kg，urea 1 kg，accessories 6%(lime 2%,gypsum 2%,calcium superphosphate 2%).Design C：corncobs 75 kg，Yingqiao residue 100 kg，cow dung 50 kg，accessories 6%(lime 2%,gypsum 2%,calcium superphosphate 2%) and Design D：corncobs 50 kg，Yingqiao residue 150 kg，cow dung 50 kg，accessories 6%(lime 2%,gypsum 2%，calcium superphosphate 2%).The dry cultivating substrate weighing over 200 kg were stacked against the wall and shaped as a semicone.After 14 days of fermentation and four times of turning-over of the stacks (respectively on the fourth day，the seventh day，the tenth day and the twelfth day)，the cultivating substrate were packed in the bags and innoculated.When the hyphae was full of the bag，it was taken out of the bag and cased with soil to make fruit developed.The results showed that the fruit body yield of Design C was the highest of the four designs，with the parametres of it as initial C/N 41.66，final C/N 23.71，T value 0.569，the bag filling ratio of innoculation 93.7%，the even bag yield of mushroom 381.9 g?kg-1，and the biological efficiency 41.0%.As the HPLC showed，the contents of chlorogenic acid and arctiin in the fermented cultivating substrate of Design C were 5.20-5.40 μg?g-1,4.49-5.30 μg?g-1 respectively and they were 0.40-0.45 μg?g-1,3.81-4.27 μg?g-1 in the fruit body.The transferring rate of arctiin from the cultivating substrate to the fruit body was high up to more than 84.93%.

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Last Update: 2017-03-22