|Table of Contents|

Study on Rapid Micropropagation Technique by Shoot Tip Culture of Ring Rot Resistant Apple Rootstock J35-8(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年13
Page:
107-110
Research Field:
Publishing date:

Info

Title:
Study on Rapid Micropropagation Technique by Shoot Tip Culture of Ring Rot Resistant Apple Rootstock J35-8
Author(s):
CHEN XiaojieGUO XingkeZHANG YuanLI ZhongyongZHANG XueyingXU Jizhong
College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001
Keywords:
apple rootstock J35-8shoot tip culturetissue culture and rapid micropropagationplant growth regulatorring rot
PACS:
S 661.103.6
DOI:
10.11937/bfyy.201513031
Abstract:
Taking the shoot tips of the ring rot resistant apple rootstock J35-8 as materials,by selecting the optimal medium,the effects of plant growth regulators on tissue-cultured seedling induction,multiplication and rooting were researched.The results showed that the basic medium was MS supplemented with sugar 35 g/L and agar 6.2 g/L,the optimal plant growth regulators of start culture medium that suited multiplication were 6-BA 1.00 mg/L+NAA 0.05 mg/L.The optimal plant growth regulators in subculture medium were 6-BA 1.0 mg/L+NAA 0.050 mg/L,and the coefficient of multiplication were exceeded 8.50 times every four weeks.The optimal plant growth regulators for rooting were IAA 1.0 mg/L+IBA 0.3 mg/L,the rooting rates of shoots were 92.68%,the average number of rooting was 9.35.Then the rooted plantlets were transplanted,the survival rates were 100.00%.

References:

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Last Update: 2015-08-21