|Table of Contents|

Study on Microspore Embryogenesis，Regeneration and Propagation in vitro on Ornamental Kale (Brassica oleracea var.acephala)(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年13
Page:
111-115
Research Field:
Publishing date:

Info

Title:
Study on Microspore Embryogenesis，Regeneration and Propagation in vitro on Ornamental Kale (Brassica oleracea var.acephala)
Author(s):
ZHU Pengfang; WANG Weizhen; LI Jun; LIU Chang; NIAN Yuxin
College of Forestry，Shenyang Agricultural University，Shenyang,Liaoning 110866
Keywords:
Brassica oleracea L.var. acephala DC.; microspore; embryogenesis; plant regeneration; propagation
PACS:
S 635.9
DOI:
10.11937/bfyy.201513032
Abstract:
Using twenty materials in ornamental kale (Brassica oleracea L.var. acephala DC.) as material,using microspore culture,influence factors of embryogenesis，plant regeneration and the subculture of multiple shoots were studied systematically.The results showed that the genotype was one of the most important factors to embryogenesis.Fifteen genotypes produced embryos with a seventy five percent induction ratio.The optimal sucrose concentration was also associated with genotypes.In different genotypes，the best ratio of 6-BA and NAA was 1∶1 or 2∶1.Compared with B5 medium，MS was better in plant regeneration.The best subculture duration shoots was 15 days with an average propagation coefficient of 10.3.

References:

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Last Update: 2015-08-21