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Cloning and Analysis of Partial Sequence of Resistance to Powdery Mildew Candidate Gene from Cucumis melon L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年19期
Page:
110-113
Research Field:
Publishing date:

Info

Title:
Cloning and Analysis of Partial Sequence of Resistance to Powdery Mildew Candidate Gene from Cucumis melon L.
Author(s):
HAN Rui; WANG Xian-lei; LI Guan
College of Life Science and Technology，Xinjiang University，Urumqi，Xinjiang 830046
Keywords:
melon; powdery mildew candidate gene; gene cloning; sequence analysis
PACS:
S 652
DOI:
-
Abstract:
Taking two high sensitive melon powdery mildew varieties ‘K413’，‘JS’ and three high resistant varieties ‘H70’，‘AN’，‘H74’ as test materials，using PCR method，part V linkage group Pm-AN was cloned and analyzed.The results showed that the cloned Pm-AN-N/C two pieces were 830 bp and 610 bp respectively from ‘K413’，‘JS’，‘H70’，‘AN’ and ‘H74’，encoding amino acids 286 and 192 respectively，homology of 99.74% and 94.00% respectively.cDNA amplification results showed that the five melon varieties appeared five different band type，respectively，appearing in two different type from ‘JS’，‘H70’，‘AN’，and all model had difference.

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Last Update: 2014-08-26