|Table of Contents|

Molecular Identification of Phytoplasma Strain Associated with Convolvulus arvensis Cluster Disease in Xinjiang(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年19期
Page:
114-117
Research Field:
Publishing date:

Info

Title:
Molecular Identification of Phytoplasma Strain Associated with Convolvulus arvensis Cluster Disease in Xinjiang
Author(s):
SHI Bao-ping; LI Cheng-liang; DU Ye-juan; XIANG Ben-chun
Key Laboratory for the Prevention and Control of Oasis Crop Disease，Shihezi University，Shihezi，Xinjiang 832000
Keywords:
Convolvulus arvensis; cluster disease; phytoplasma; molecular identification
PACS:
Q 813.1
DOI:
-
Abstract:
In order to identify the taxonomic status of phytoplasma of Convolvulus arvensis cluster disease in Xinjiang，using phytoplasma universal primers P1/P7 and R16F2n/R16R2 for 16S rRNA gene to detect phytoplasma in Convolvulus arvensis showing symptoms of cluster disease in Xinjiang Shihezi area.The target fragment was amplified from the total DNA by nested-PCR，then it was cloned and sequenced.The results showed that the 16S rRNA gene of Convolvulus arvensis cluster phytoplasma consists of 1 229 bp (Genbank No.:KC414725)，it clustered together with the members of phytoplasma Peanut witches’broom (16SrII) group，and shared a high similarity(98％)with the phytoplasma EBB-A (Eggplant big bud） belongs to 16SrII.So it was confirmed that the phytoplasma strain Convulus arvensis cluster disease was the member of phytoplasma 16SrII group.

References:

[1]王真辉,陈秋波,郭志立,等.假臭草丛枝病植原体16S rDNA检测与PCR-RFLP分析[J].热带作物学报,2007,28(4)：52-56.

[2]蔡红,孔宝华,陈海如.长春花黄化植原体(PY)株系的检测与鉴定[J].微生物报,2003,43(1):116-119.

[3]廖晓兰,朱水芳,罗宽.植原体的分类及分子生物学研究进展[J].植物检疫,2002,16(3):167-172.

[4]Lim P O，Sears B B.16S rRNA sequence indicates that plant-pathogenic mycoplasma like organisms are evolutionarily distinct from animal mycoplasmas[J].Journal of Bacteriology,1989,171:5901-5906.

[5]石峰,杨伟俊,于睿.田旋花药材质量标准的研究[J].时珍国医国药,2009,20(10):2542-2543.

[6]罗燕娜,杜娟,李俊华.棉苗立枯病拮抗菌的筛选及鉴定[J].石河子大学学报(自然科学版),2010,28(5):556-560.

[7]秦荣,张伟,刘炜炜.dsRNA介导的双价抗病毒沉默载体的构建[J].石河子大学学报(自然科学版),2012,30(4):430-433.

[8]Ahrens U，Seeüller E.Detection of DNA of plant pathogenic mycoplasma like organisms by a polymerase chain reaction that amplifies a sequence of the 16SrRNA gene[J].Phytopathology,1992,82:828-832.

[9]李正男,张磊,吴云锋.丁香卷叶病植原体的分子鉴定[J].植物病理学报,2012,42(2):131-138.

[10]Costa A S.Duas novas molesti as devirus do tomate iroem Sao Paulo[J].Biological，1949，15：79-81.

[11]任娟,孙佰平,杜娟.枯草芽孢杆菌S44菌株ituD基因的克隆及原核表达[J].石河子大学学报(自然科学版),2012,30(5):578-581.

[12]Lee I M，Gundersen Rindal D E，Davis R E，et al.Revised classification scheme of phytoplasma based on RFLP analyses of l6S rRNA and ribosomal protein gene sequences[J].International Journal of Systematic Bacteriology,1998,48:1153-1169.

[13]秦国夫,赵俊,刘小勇.植原体分子分类的现状与存在的问题[J].林业科学,2002,38(6):125-136.

[14]Zhao Y，Sun Q R，Wei W，et al.‘Candidatus Phytoplasma tamaricis’，a novel taxon discovered in witches’ broom diseased salt cedar (Tamarix chinensis Lour.)[J].International Journal of Systematic and Evolutionary Microbiology,2009,59:2496-2504.

[15]顾沛雯,安凤秋,吴云峰,等.小麦蓝矮病植原体16S rDNA基因片段的比较分析[J].植物病理学报,2005,3(5):403-411.

[16]Lee I M，Davis R E，Gundersen D E.Phytoplasma：phytopathogenic mollicutes[J].Annual Review of Microbiology,2000,54:221-255.


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Last Update: 2014-08-26