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StudyontheTransformationofCs-FAD7GeneintoNicotianatabacum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年11期
Page:
120-123
Research Field:
Publishing date:

Info

Title:
StudyontheTransformationofCs-FAD7GeneintoNicotianatabacum
Author(s):
LIUChun-xiang; LENGChuan-yuan; LIUFu-yan
WeifangUniversity，KeyLaboratoryofBiochemistryandMolecularBiologyofUniversityinShandongProvince,Weifang,Shandong261061
Keywords:
Nicotianatabacum; Agrobacteriumtumefaciens; cucumber; FAD7gene
PACS:
S572.035.2
DOI:
-
Abstract:
Using‘GexinNo.1’tobaccoseedsastestmaterials,FAD7genewastransferredintotobaccoleaveswiththehelpofAgrobacteriumtumefaciens.ShootswereregeneratedonMSmediumsupplementedwith300mg/LAmpicillin，1.0mg/L6-BAand0.1mg/LNAAandrootedonMSmediumwith300mg/LAmpicillinand100mg/LKanamycin.PCRanalysisprovedFAD7geneintegrationintoKanamycinresistanceplants.TheresultsofPCRanalysisinT1generationtransgenicplantsshowedthatthetransformedgenecouldinherittonextgeneration，itgrewwellunderlowtemperature.TheparametersofleavechlorophyllfluorescenceF0decreasedslightly，andthevaluesofFv/FmkepthighlevelafterCsFAD7genetransformedintotobacco.fattyacidanalysisshowedthatthecontentsratiooflinolenicacidinleaveswerehigherintransgenictobaccothaninwildtypetobacco.

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Last Update: 2014-10-09