«Previous Article|Table of Contents|Next Article»

¡¶±±·½Ô°ÒÕ¡·[ISSN:1001-0009/CN:23-1247/S]

Issue:

2019Äê06

Page:

18-27

Research Field:

Publishing date:

Info

Title:

Isolation and Investigation of Glutathione S-transferase Genes in Tibetª« Rhodiola renulataª«

Author(s):

ZHANG Lipeng; YU Deshui; TENG Yanjiao; CHEN Chengbin; SONG Wenqin

(College of Life Science,Nankai University,Tianjin 300071)

Keywords:

Rhodiola crenulataª«; GST; bioinformation; qRT-PCR; expression pattern

PACS:

-

DOI:

10.11937/bfyy.20183024

Abstract:

The glutathione S-transferase (GST) gene family members were analysis by bioinformation and qRT-PCR for Tibet ª«Rhodiola crenulataª«£¬in order to lay a foundation for the further studies of function about GST family genes in ª«Rhodiolaª«.The results showed that 11ª« RcGSTsª« genes were classified into six subgroups according to protein sequence identity contained tau (ª«RcGSTU1,RcGSTU2,RcGSTU3ª«),phi (ª«RcGSTF1,RcGSTF2,RcGSTF3,RcGSTF4ª«),lambd (ª«RcGSTLª«),microsomal (ª«RcGSTMª«),theta (ª«RcGST1ª«),zeta (ª«RcGSTZª«).All ª«RcGSTsª« genes located in cytoplasm and without transmembrane domain expectª« RcGSTMª«.The expression patterns for tissue and stresses presented thatª« RcGSTsª« had different tissue expression specificity.Under cold stress,4 of 11 ª«RcGSTsª« expression levels were up-regulation and 7 of 11 ª«RcGSTsª« were down-regulation.For abscisic acid stresses,all ª«RcGSTsª« were induced the expression levels.

References:

[1]HAYES J D,PULFORD D J.The glutathione S-transferase supergene family:Regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance[J].Crc Critical Reviews in Biochemistry,1995,30(6):521-600.[2]HAN X M,YANG Z L,LIU Y J,et al.Genome-wide profiling of expression and biochemical functions of the ª«Medicago glutathione ª«S-transferase gene family[J].Plant Physiol Biochem,2018,126:126-133.[3]DIXON D P,EDWARDS R.Roles for stress-inducible lambda glutathione transferases in flavonoid metabolism in plants as ª©identified by ligand fishing[J].Journal of Biological Chemistry,2010,285(47):36322-36329.[4]LAN T,YANG Z L,XUE Y,et al.Extensive functional ª©diversification of the ª«Populusª« glutathione S-transferase supergene family[J].Plant Cell,2009,21(12):3749-3766.[5]OAKLEY A J.Glutathione transferases:New functions[J].Current Opinion in Structural Biology,2005,15(6):716-723.[6]DU J,REN J,YE X,et al.Genome-wide identification and expression analysis of the glutathione S-transferase (GST) family under different developmental tissues and abiotic stresses in ª©Chinese cabbage (ª«Brassica rapaª« ssp.pekinensis)[J].PeerJ Preprints,2018(6):1-23.[7]ABDUL KAYUM M,NATH U K,PARK J I,et al.Genome-wide identification,characterization,and expression profiling of glutathione S-transferase (GST) family in pumpkin reveals likely role in cold-stress tolerance[J].Genes (Base l),2018,9(2):1-21.[8]LABROU N E,PAPAGEORGIOU A C,PAVLI O,et al.Plant GSTome:Structure and functional role in xenome network and plant stress response[J].Curr Opin Biotechnol,2015(32):186-194.[9]ROXAS V P,LODHI S A,GARRETT D K,et al.Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione ª©peroxidase[J].Plant & Cell Physiology,2000,41(11):1229.[10]ROXAS V P,JR S R,ALLEN E R,et al.Overexpression of glutathione S-transferase/glutathione peroxidase enhances the growth of transgenic tobacco seedlings during stress[J].Nature Biotechnology,1997,15(10):988.[11]½­¶­Àö,²Å»ª,¶Ëľ»Û×Ó,µÈ.´ó¶¹ª«GSTª«»ùÒò¼Ò×åÈ«»ùÒò×éɸѡ¡¢·ÖÀàºÍ±í´ï[J].·Ö×ÓÖ²ÎïÓýÖÖ,2013,4(5):465-475.[12]³ÉÑåÀö,ÍõêØ,˾ÎÀ½Ü,µÈ.¶ìÕÆé±Êôª«GSTª«¼Ò×å»ùÒòµÄ¿Ë¡Óë±í´ï·ÖÎö[J].Î÷±±Ö²Îïѧ±¨,2018(3):445-455.[13]½ðÑ©»¨,ºéÑÞ,»ÆºÓ,µÈ.¹ÏÒ¶¾Õ¹Èë׸ÊëÄתÒÆø»ùÒòª«GSTª«µÄ·ÖÀë¼°±í´ï·ÖÎö[J].Ô°ÒÕѧ±¨,2013,40(6):1129-1138.[14]ÕÅÑÇÕæ,Τ¿µ,ÍõÀöÔ§,µÈ.»ùÓÚת¼×é²âÐò¶Ô²èÊ÷ª«GSTª«»ùÒò±í´ïµÄÑо¿[J].²èÒ¶¿Æѧ,2016,36(5):513-522.[15]ZHANG J Q,MENG S Y,WEN J,et al.DNA barcoding of ª«Rhodiolaª« (Crassulaceae):A case study on a group of recently diversified medicinal plants from the Qinghai-Tibetan Plateau[J].PLoS One,2015,10(3):1-15.[16]¹ú¼ÒÒ©µäίԱ»á.ÖйúÒ©µä£ºÒ»²¿[M].±±¾©:ÖйúÒ½Ò©¿Æ¼¼³ö°æÉç,2015:154.[17]LAI M C,LIN J G,PAI P Y,et al.Effects of ª«Rhodiola crenulataª« on mice hearts under severe sleep apnea[J].BMC Complement Altern Med,2015(15):198.[18]QU Z Q,ZHOU Y,ZENG Y S,et al.Protective effects of aª« Rhodiola crenulataª« extract and salidroside on hippocampal neurogenesis against streptozotocin-induced neural injury in the rat[J].PLoS One,2012,7(1):1-17.[19]KOSANOVIC D,TIAN X,PAK O,et al.Rhodiola:An ordinary plant or a promising future therapy for pulmonary hypertension? a brief review[J].Pulm Circ,2013,3(3):499-506.[20]ZHANG J Q,MENG S Y,WEN J,et al.Phylogenetic relationships and character evolution of ª«Rhodiola ª«(Crassulaceae) based on nuclear ribosomal ITS and plastid trnL-F and psbA-trnH sequences[J].Systematic Botany,2014,39(2):441-451.[21]FU Y,LI L,HAO S,et al.Draft genome sequence of the ª©Tibetan medicinal herb ª«Rhodiola ª©crenulataª«[J].Gigascience,2017,6(6):1-5.[22]LAN X,CHANG K,ZENG L,et al.Engineering salidroside biosynthetic pathway in hairy root cultures of ª«Rhodiola crenulataª« based on metabolic characterization of tyrosine decarboxylase[J].PLoS One,2013,8(10):e75459.[23]ZHANG J Q,MENG S Y,ALLEN G A,et al.Rapid radiation and dispersal out of the Qinghai-Tibetan Plateau of an alpine plant lineageª« Rhodiolaª« (Crassulaceae)[J].Mol Phylogenet Evol,2014,77:147-158.[24]YANG M,ZHANG Y,ZHANG H,et al.Identification of ª«MsHsp20ª« gene family inª« Malus sieversiiª« and functional characterization ofª« MsHsp16.9ª« in heat tolerance[J].Front Plant Sci,2017(8):1761.[25]ÕÔ·çÖÎ,ÍõÑ©Ó¨,ÍõÐÀÀÙ,µÈ.»¨Ò¬²Ëª«BoPGIP2ª«»ùÒòµÄ¿Ë¡Óë±í´ïˮƽ·ÖÎö[J].±±·½Ô°ÒÕ,2016(7):77-84.[26]ÕÅÁ¦Åô,ÕÅÒøÐË,ËÎÎÄÇÛ,µÈ.ºì¾°ÌìÊôÖ²ÎïҶƬRNA¸ßЧÌáÈ¡µÄ·½·¨[J].ÄÏ¿ª´óѧѧ±¨(×ÔÈ»¿Æѧ°æ),2017(6):48-53.[27]WEI T,DENG K,GAO Y,et al.ª«Arabidopsis DREB1Bª« in transgenic ª«Salvia miltiorrhizaª« increased tolerance to drought stress without stunting growth[J].Plant Physiol Biochem,2016,104:17-28.[28]WEI T,DENG K,LIU D,et al.Ectopic expression of DREB transcription factor,ª«AtDREB1Aª«,confers tolerance to drought in transgenic ª«Salvia miltiorrhizaª«[J].Plant Cell Physiol,2016,57(8):1593-1609.[29]WEI T,DENG K,ZHANG Q,et al.Modulating ª«AtDREB1Cª« expression improves drought tolerance in ª«Salvia miltiorrhizaª«[J].Front Plant Sci,2017(8):52.[30]MUHAMMAD K,AHMAD W.A novel L-ascorbate peroxidase 6 gene,ª«ScAPX6ª«,plays an important role in the regulation of response to biotic and abiotic stresses in sugarcane[J].Frontiers in Plant Science,2017(8):1-13.[31]GAO F,CHEN J,MA T,et al.The glutathione peroxidase gene family in ª«Thellungiella salsugineaª«:Genome-wide identification,classification,and gene and protein expression analysis under stress conditions[J].Int J Mol Sci,2014,15(2):3319-3335.[32]BINH LT,OONO K.Molecular cloning and characterization of genes related to chilling tolerance in rice[J].Plant Physiology,1992,99(3):1146-1150.[33]EDWARDS R,DIXON D P,WALBOT V.Plant glutathione S-transferases:Enzymes with multiple functions in sickness and in health[J].Trends in Plant Science,2000,5(5):193-198.[34]³ÂÃ÷»Ô,ÕÅ־¼,١ΰ˪,µÈ.µÍÎÂвÆÈÏÂÍÑÂäËá¶Ô½ðîÎʯõúĤ͸ÐÔºÍÄÚÔ´¼¤ËصÄÓ°Ïì[J].±±·½Ô°ÒÕ,2015(23):74-78.[35]½Ãô,½¹óÇå.ÍÑÂäËáÓëÖ²Îï·ÇÉúÎïÄæ¾³¿¹ÐÔÑо¿½øÕ¹[J].±±·½Ô°ÒÕ,2014(8):184-188.[36]ÕÅéª,ºéÓÀ´Ï,ÍõÓñ,µÈ.ÍÑÂäËáºÍË®ÑîËá¶ÔÔ½¶¬ÆÚ²èÊ÷ҶƬ¿¹º®ÉúÀíÖ¸±êµÄÓ°Ïì[J].±±·½Ô°ÒÕ,2010(22):21-24.[37]ÍõÇ¿,ÈîÏþ,ÀîºÉµÏ,µÈ.ÕäÏ¡Ò©ÓÃ×ÊÔ´Ö²Îïºì¾°ÌìÑо¿ÏÖ×´¡¢ÎÊÌâÓë¶Ô²ß[J].×ÔÈ»×ÊԴѧ±¨,2007,22(6):880-889.[38]ÍõÇ¿,ÈîÏþ,·½À¼,µÈ.×ÊÔ´Ö²Îïºì¾°ÌìÑо¿×ÛÊö[J].н®Å©Òµ´óѧѧ±¨,2002,25(4):57-62.[39]GRECH-BARAN M,SYKLOWSKA-BARANEK K,PIETROSIUK A.Biotechnological approaches to enhance salidroside,rosin and its derivatives production in selected ª«Rhodiolaª« spp.ª«in vitroª« cultures[J].Phytochem Rev,2015,14(4):657-674.[40]XIN J,ZHANG R C,WANG L,et al.Researches on transcriptome sequencing in the study of traditional Chinese medicine[J].Evid Based Complement Alternat Med,2017(1):1-7.[41]SORANZO N,GORLA MS,MIZZI L,et al.Organisation and structural evolution of the rice glutathione S-transferase gene family[J].Molecular Genetics & Genomics,2004,271(5):511-521.[42]JAKOBSSON P J,MORGENSTERN R,MANCINI J,et al.Common structural features of mapeg:A widespread superfamily of membrane associated proteins with highly divergent functions in eicosanoid and glutathione metabolism[J].Protein Science,2010,8(3):689-692.[43]MALNOY M,REYNOIRD J P,BOREJSZAWYSOCKA E E,et al.Activation of the pathogen-inducible Gst1 promoter of potato after elicitation by ª«Venturia inaequalis ª«andª« Erwinia amylovoraª« in transgenic apple (ª«Malus¡Ádomesticaª«)[J].Transgenic Research,2006,15(1):83-93.[44]öÃÑÅæº,Àî½Ü,ÕÅˬ,µÈ.Ö²ÎïÆô¶¯×ÓÑо¿½øÕ¹[J].±±·½Ô°ÒÕ,2015(22):186-189.[45]QIU P.Recent advances in computational promoter analysis in understanding the transcriptional regulatory network[J].Biochemical and Biophysical Research Communications,2003,309(3):495-501.[46]FALTIN Z,HOLLAND D,VELCHEVA M,et al.Glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation[J].Plant Cell Physiol,2010,51(7):1151-1162.[47]SUZUKI N,KOUSSEVITZKY S,MITTLER R,et al.ROS and redox signalling in the response of plants to abiotic stress[J].Plant Cell Environ,2012,35(2):259-270.[48]MILLER G,SHULAEV V,MITTLER R.Reactive oxygen signaling and abiotic stress[J].Physiol Plant,2008,133(3):481-489.[49]ZHAO G R,ZHANG H M,YE T X,et al.Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B[J].Food & Chemical Toxicology,2008,46(1):73-81.[50]LIU H,WANG X,WANG D,et al.Effect of drought stress on growth and accumulation of active constituents in ª«Salvia miltiorrhiza ª«Bunge[J].Industrial Crops and Products,2011,33(1):84-88.

Memo

Memo:

-

Common functions

Last Update: 2019-04-03