|Table of Contents|

Genetic and Related Gene Expression Analysis of Leaf Marginal Traits in Brassica juncea

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年06
Page:
12-17
Research Field:
Publishing date:

Info

Title:
Genetic and Related Gene Expression Analysis of Leaf Marginal Traits in Brassica juncea
Author(s):
YU Chen1; YUAN Zhenzhen2; SHA Aihua1; WAN Zhengjie2
(1.Agricultural College,Yangtze University,Jingzhou,Hubei 434000;2.College of Horticulture and Forestry,Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology,Ministry of Education,Wuhan,Hubei 430070)
Keywords:
Brassica juncea; leaf margin nicking; genetic analysis; gene expression
PACS:
-
DOI:
10.11937/bfyy.20182737
Abstract:
Two Brassica juncea mustard materials with leaf margin and leaf margin serration were used as materials,the F2 and BC1 segregating populations were constructed,the splicing traits of individual plants were investigated and qRT-PCR analysis of related genes was performed in order to clarify the inheritance of leaf margin traits of leaf mustard and provide theoretical basis for Brassica juncea breeding.The results showed that the leaf margin of the mustard leaf was controlled by a pair of dominant genes,and the gene was named BjLB (Leaf-lobe in Brassica juncea);qRT-PCR analysis showed that the expression level of [STBX]KNAT1[STBZ] gene in the leaf margin was higher than that of the leaf margin,and the other genes difference expressed in the two parents were not obvious,and it was speculated that the [STBX]KNAT1[STBZ] gene might be a candidate gene.

References:

[1]万正杰，李海渤，姚培杰，等.芥菜类蔬菜杂种优势利用的研究进展与展望[J].华中农业大学学报，2018,37(1):115-120.[2]SHYAM P.Brassica cytogenetics:A historical journey and my personal reminiscence[J].Chinese journal of oil crop sciences,2010,32(1):163-172.[3]涂金星，傅廷栋．甘蓝型油菜几个苗期形态标记的遗传[J]．华中农业大学学报，2001(20)：318-320.[4]SHANI E,BEN-GERA H,SHLEIZER-BURKO S,et al.Cytokinin regulates compound leaf development in tomato[J].Plant Cell,2010(22):3206-3217.[5]BILSBOROUGH G D,RUNIONS A,BARKOULAS M,et al.Model for the regulation of Arabidopsis thaliana leaf margin development[J].P Natl Acad SciUSA,2011,108:3424-3429.[6]FLEISHON S,SHANI E,ORI N,et al.Negative reciprocal interactions between gibberellin and cytokinin in tomato[J].New Phytol,2011,190:609-617.[7]YANAI O,SHALLI E,RUSS D,et al.Gibberellin partly mediates LANCEOLATE activity in tomato[J].Plant J,2011,68(4):571-82.[8]曾国平，曹寿椿．不结球白菜主要经济性状遗传规律的研究Ⅰ、11个质量性状的遗传分析[J]．南京农业大学学报，1996，19(3):23-30.[9]刘静.萝卜败蕾的细胞形态学和小白菜裂叶性状分子标记研究[D].杨凌：西化农林科技大学，2008.[10]葛颂，洪德元．泡沙参复合体(桔梗科)的物种生物学研究III:性状的遗传变异及其分类价值[J].植物分类学报，1995，33(5):433-443.[11]王玉刚，李萌，吕晓雯，等．白菜叶裂数性状主基因＋多基因遗传分析[J]．西北植物学报，2012，32(2):252-256.[12]刘义华，冷蓉，张召荣，等．茎瘤芥叶片刺毛与缺刻的遗传分析[J]．园艺学进展（第七辑），2006(1):666-669.[13]LI F,KITASHIBA H,INABA K et al.A Brassica rapa linkage map of EST-based SNP markers for identification of candidate genes controlling flowering time and leaf morphological traits[J].DNA Research,2009(16):311-323.[14]LOU P,ZHAO J,KIM J S,et al.Quantitative trait loci for flowering time and morphological traits in multiple populations of Brassica rapa[J].Journal of Experimental Botany,2007,58:4005-4016.[15]邓杰，王辉，程锋，等.控制大白菜和白菜型油菜叶缘裂刻的 QTL 定位及分析[J]．园艺学报，2012，39(4)：661-668.[16]ZHAO J J，PAULO M J，JAMAR D，et al.Association mapping of leaf traits flowering time and phytate content in Brassica rapa[J].Genome，2007,50：963-973.[17]FENG L，KITASHIBA H，INABA K，et al.A Brassica rapa linkage map of EST-based SNP markers for identification of candidate genes controlling flowering time and leaf morphological traits[J].DNA Research，2009(16):311-323.[18]李婉莎，王春涛，胡向阳．拟南芥叶边缘锯齿状突变体的分离与鉴定[J]．植物分类与资源学报，2012，34(1)：28-32.[19]BYRNE M,TIMMERMANS M,KIDNER C,et al.Development of leaf shape[J].Curr Opin Plant Biol,2001(4):38-43.[20]GUO M,THOMAS J,COLLINS G,et al.Direct repression of KNOX loci by the asymmetric leaves complex of Arabidopsis[J].Plant Cell,2008(20):48-58.[21]HAKE S,SMITH H M S,HOLTAN H,et al.The role of KNOX genes in plant development[J].Annu Rev Cell Dev Biol,2004(20):125-151.[22]HAY A,BARKOULAS M,TSIANTIS M.Asymmetric leaves and auxin activities converge to repress brevipedicellus expression and promote leaf development in Arabidopsis[J].Develo Pment,2006,133:3955-3961.[23]UCHIDA N,KIMURA S,KOENIG D,et al.Coordination of leaf development via regulation of KNOXI genes[J].J Plant Res,2010,123:7-14.[24]VOLLBREEHT E,VEIT B,SINHA N,et al.The developmental gene knotted-1is a member of a maize homeobox gene family[J].Nature,1991,350:241-243.[25]余四斌.分子遗传图谱的构建[M].北京:科学出版社,2004:403-440.[26]霍雪萍，张琳萍，赵向绒，等.RNA 质量对内参基因选择和 qRT-PCR 结果评价的影响[J].生物技术通讯，2018,29(3):415-418.

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Last Update: 2019-04-03