|Table of Contents|

Molecular Identity Establishment of Several Apricot Strains Based on Fluorescent Labeled SSR Markers(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年04
Page:
34-40
Research Field:
Publishing date:

Info

Title:
Molecular Identity Establishment of Several Apricot Strains Based on Fluorescent Labeled SSR Markers
Author(s):
YUAN KejunNIU QinglinGE FurongWANG JiangyongWANG Peijiu
Shandong Institute of Pomology,Tai′an,Shandong 271000
Keywords:
apricotgermplasmSSR markerfluorescent labeled primermolecular identity
PACS:
-
DOI:
10.11937/bfyy.20172966
Abstract:
The 15 germplasms (including 4 new strains) and 6 SSR markers with fluorescent labeled primers were used to perform PCR experiment,the molecular identities of 4 strains selected from hybrid apricot plants were established.The purpose of this study was to provide experiment basis for cultivar right protection of 4 apricot strains using molecular identification.The results showed that a total of 47 polymorphic alleles and 57 electrophoresis patterns were revealed by the 6 markers,with an average of 7.8 alleles and 9.8 patterns for each marker;and the molecular identities of 4 strains were different from each other and other 11 germplasms.These results provided experiment basis for cultivar right protection of 4 apricot strains using molecular identification.

References:

[1]张加延.中国李杏资源及开发利用研究[M].北京:中国林业出版社,1999:19-23.

[2]苑克俊,牛庆霖.杏及其加工制品的质量安全与产业发展[J].食品科学技术学报,2015,33(4):6-10,15.
[3]山东省果树研究所.山东果树志[M].济南:山东科学技术出版社,1999:247-276.
[4]MORGANTE M,OLIVIERI A.PCR-amplified microsatellites as markers in plant genetics[J].The Plant Journal,1993,3(1):175-182.
[5]DANGL G S,YANG J,GOLINO D A,et al.A practical method for almond cultivar identification and parental analysis using simple sequence repeat markers[J].Euphytica,2009,168:41-48.
[6]MORIYA S,IWANAMI H,OKADA K,et al.A practical method for apple cultivar identification and parent-offspring analysis using simple sequence repeat markers[J].Euphytica,2011,177(1):135-150.
[7]艾呈祥,张力思,魏海蓉,等.甜樱桃品种SSR指纹图谱数据库的建立[J].中国农学通报,2007,23(5):55-58.
[8]陈昌文,曹珂,王力荣,等.中国桃主要品种资源及其野生近缘种的分子身份证构建[J].中国农业科学,2011,44(10):2081-2093.
[9]王立新,张小军,史星雲,等.苹果栽培品种SSR指纹图谱的构建[J].果树学报,2012,29(6):971-977.
[10]高源,田路明,刘凤之,等.利用SSR荧光标记构建92个梨品种指纹图谱[J].园艺学报,2012,39(8):1437-1446.
[11]高源,刘凤之,王昆,等.苹果部分种质资源分子身份证的构建[J].中国农业科学,2015,48(19):3887-3898.
[12]杜晶晶,刘国银,魏军亚,等.基于SSR标记构建葡萄种质资源分子身份证[J].植物研究,2013,33(2):232-237.
[13]张靖国,田瑞,陈启亮,等.基于SSR标记的梨栽培品种分子身份证的构建[J].华中农业大学学报,2014,33(1):12-17.
[14]李国田,张美勇,相昆,等.基于ISSR标记的16个核桃品种遗传多样性分析及分子身份构建[J].核农学报,2015,29(10):1884-1892.
[15]程本义,夏俊辉,龚俊义,等.SSR荧光标记毛细管电泳检测法在水稻DNA 指纹鉴定中的应用[J].中国水稻科学,2011,25 (6):672-676.
[16]徐雷锋,葛亮,袁素霞,等.利用荧光标记SSR构建百合种质资源分子身份证[J].园艺学报,2014,41(10):2055-2064.
[17]ACHTAK H,OUKABLI A,ATER M,et al.Microsatellite markers as reliable tools for fig cultivar identification[J].Journal of the American Society for Horticultural Science,2009,134(6):624-631.
[18]麻丽颖,孔德仓,刘华波,等.36份枣品种SSR指纹图谱的构建[J].园艺学报,2012,39(4):647-654.
[19]何天明,陈学森,高疆生,等.新疆栽培杏群体遗传结构的SSR分析[J].园艺学报,2006,33(4):809-812.
[20]张淑青,刘冬成,刘威生,等.普通杏品种SSR遗传多样性分析[J].园艺学报,2010,37(1):23-30.
[21]王玉安,欧巧明,陈建军,等.甘肃地方杏品种资源的SSR遗传多样性分析[J].西北农业学报,2013,22(3):98-100.
[22]DIRLEWANGER E,COSSON P,TAVAUD M,et al.Development of microsatellite markers in peach[(Prunus persica L.) Batsch]and their use in genetic diversity analysis in peach and sweet cherry(Prunus avium L.)[J].Theoretical and Applied Genetics,2002,105:127-138.
[23]DONDINI L,LAIN O,GEUNA F,et al.Development of a new SSR-based linkage map in apricot and analysis of synteny with existing Prunus maps[J].Tree Genetics & Genomes,2007(3):239-249.
[24]苑克俊,王长君,王培久,等.赤霉素处理日光温室杏种子对加速育苗进程的效果研究[J].山东农业科学,2013,45(10):76-78.
[25]苑克俊,王长君,王培久,等.杏采后当年播种培育育种植株技术研究[J].天津农业科学,2014,20(11):88-92.

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Last Update: 2018-02-23