|Table of Contents|

Selection of Pollen Culture in vitro Conditions and Pollen Viability in Armeniaca vulgaris var .ansu

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年04
Page:
26-33
Research Field:
Publishing date:

Info

Title:
Selection of Pollen Culture in vitro Conditions and Pollen Viability in Armeniaca vulgaris var .ansu
Author(s):
MIAO Yang1; DONG Shengjun1; WU Yueliang1; LIU Mingguo1; LI Huan2; CHEN Jianhua1
1.College of Forestry,Shenyang Agricultural Uinversity,Shengyang,Liaoning 110866;2.Ecological Experimental Forest Farm,Beipiao,Liaoning 122100
Keywords:
Armeniaca vulgaris var.ansu; pollen culture in vitro; pollen viability
PACS:
-
DOI:
10.11937/bfyy.20173199
Abstract:
Excellent families and clones of Armeniaca vulgaris var.ansu were used as the study objects.The method of pollen culture in vitro was used to study the pollen culture in vitro conditions and pollen vitality of Armeniaca vulgaris var.ansu.In order to provide theoretical basis for selecting the male parent plants in cross breeding of Armeniaca vulgaris var.ansu.The results showed that sucrose concentration and incubation time in the medium components were important factors affecting the pollen viability.The optimum medium of Armeniaca vulgaris var.ansu pollen culture in vitro was 10% sucrose,0.005% boric acid and cultured for 6.0 hours.It could be divided into four growth periods according to the pollen tubes growth rate,the pollen germination period was 0.3-1.0 hour,the rapid growth period was 1.0-2.5 hours,the slowing growth period was 2.5-6.0 hours and the lag growth period was >6.0 hours.All of Armeniaca vulgaris var.ansu families and clones for the normal pollen fertility.The pollen viability of No.209 family was the strongest.Its pollen germination rate and the length of pollen tube were respectively 81.33%,1 136.14 μm.The pollen germination rate of No.J118 clone was the highest and with the percent of 63.35.The length of its pollen tube was the longest and with the average length of 1 043.78 μm.There was no correlation between pollen germination rate and pollen tube length of Armeniaca vulgaris var.ansu families.The clones were significantly positively correlated.The pollen germination rate of Armeniaca vulgaris var.ansu families were significantly better than clones.The pollen vitality of three families were significantly better than Armeniaca sibirica family.

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Last Update: 2018-02-23