|Table of Contents|

Diversity Analysis of Retrotransposon Reverse Transposases From Wild Blueberry Vaccinium uliginosum L.in Greater Khingan(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年04
Page:
41-48
Research Field:
Publishing date:

Info

Title:
Diversity Analysis of Retrotransposon Reverse Transposases From Wild Blueberry Vaccinium uliginosum L.in Greater Khingan
Author(s):
ZHANG Wenbo; BAI Yang; YU Limin; FU Hongfeng; YAN Wei
College of Forestry,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019
Keywords:
Vaccinium uliginosum; reverse transposase; retrotransposon; genetic diversity
PACS:
-
DOI:
10.11937/bfyy.20172738
Abstract:
For analyzing genetic diversity and empoldering credible molecular marker of wild blueberry, Vaccinium uliginosum in Greater Khingan,reverse transposases of Ty3-Gypsy-like retrotransposons were isolated using homology based cloning techniques,and homology and phylogenetic relation were analyzed.The results revealed that the length of the nucleotide sequences of the 19 isolated sequences （named as VuGRE1-19) varied from 376 bp to 418 bp with homology ranged from 19.8%-97.8%,showing a high heterogeneity.Sequence analysis for the VuGRE1-19 showed that the stop code mutation with a high frequency occured in eight sequences,suggesting terminator mutation was the major reason resulted in heterogeneity of retrotransposons in V.uliginosum.Phylogenetic analysis showed that the 19 of sequences are consisted of two families,Family I contained only 3 sequences which showed low homology between each other,and far relationships with reverse transposases from the other plants,revealing ancient origin and low transcriptional activity.Family II contained 16 sequences,which consisted of 3 Subfamilies,the sequences of the Subfamilies show high similarity and close relationships between each other.The sequences of Subfamily 3 had a closer relationship with reverse transposases from the other plants,such as Malus pumila,Larix gmelinii,Peperomia caperata,Vigna radiate,Prunus salicina and Eleocharis dulcis,indicating extensive transfer of the subfamily members among different plant species.These results suggested that the Family II retrotransposons widespreadly existed in genome and played an important role in adaptive genetic evolution of the V.uliginosum.

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Last Update: 2018-02-24