|Table of Contents|

Identification of a Cordyceps cicadae Strain Based on ITS Sequences and Production of Fibrinolytic Enzymes by the Fungus

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年03
Page:
28-33
Research Field:
Publishing date:

Info

Title:
Identification of a Cordyceps cicadae Strain Based on ITS Sequences and Production of Fibrinolytic Enzymes by the Fungus
Author(s):
LONG Liangkun1; LIANG Zihao1; LIN Qunying2; HU Yi1; WU Wenjie1; DING Shaojun1
1.College of Chemical Engineering,Nanjing Forestry University,Nanjing,Jiangsu 210037;2.Nanjing Institute for the Comprehensive Utilization of Wild Plants,All China Federation of Supply and Marketing Cooperatives,Nanjing,Jiangsu 210042
Keywords:
Cordyceps cicadae; fibrinolytic enzyme; nutritional sources; liquid culture
PACS:
-
DOI:
10.11937/bfyy.20171627
Abstract:
A newly isolated Cordyceps cicadae strain CC1504 was used as research material in this study.The taxonomy position of the fungus was confirmed by analysis of its internal transcribed spacer （ITS) sequences of ribosomal DNA.Agarose-fibrin plate method was employed to detect the fibrinolytic enzymes activity produced by the fungal strain.The carbon or nitrogen sources for the production of fibrinolytic enzymes were screened by single-factor experiments.Orthogonal experimental design was conducted to optimize the medium component for fibrinolytic enzymes production.The results indicated that the fungal strain CC1504 belonged to Cordyceps cicadae according to the phylogenetic tree analysis based on ITS sequences.The optimal carbon source was lactose for the production of fibrinolytic enzymes,and the highest production level up to 248.95 U?mL-1,followed by glycerol,given the yield at 198.94 U?mL-1.Peptone was the optimal nitrogen source for the production of enzymes,giving maximum level at 240.68 U?mL-1,and followed by yeast extract with production level of 89.23 U?mL-1.The optimized medium formula （carbon and nitrogen sources) was as:lactose 10 g?L-1,peptone 10 g?L-1 and yeast extract 3 g?L-1,giving the production of 262.05 U?mL-1.

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Last Update: 2018-02-09