|Table of Contents|

Optimizing Fermentation Condition for Antagonistic JM-3 Strain and Protection Effect Against Valsa ceratosperma

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年04
Page:
54-59
Research Field:
Publishing date:

Info

Title:
Optimizing Fermentation Condition for Antagonistic JM-3 Strain and Protection Effect Against Valsa ceratosperma
Author(s):
ZHANG Shuying1QU Tianli2ZHAO Chuande1
1.College of Plant Protection,Qingdao Agricultural University,Qingdao,Shandong 266109;2.College of Chemistry and Pharmaceutical Sciences,Qingdao Agricultural University,Qingdao,Shandong 266109
Keywords:
antifungal substancesfermentation conditiondisease protectionValsa ceratosperma
PACS:
-
DOI:
10.11937/bfyy.20171622
Abstract:
Strain JM-3 isolated from sisal was used as study object,the single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium and fermentation conditions of JM-3,and the control effect on apple excised twigs against Valsa ceratosperma was tested,in order to provide reference for establishing industrial production.The results showed that optimum medium was glucose 30 g?L-1,peptone 20 g?L-1,K2HPO4 1.0 g?L-1.The optimal fermentation conditions were initial pH 7,medium capacity 150 mL or 100 mL/250 mL,temperature 33 ℃,fermentation time 120 hours.Inhibitory activity of strain JM-3 fermentation solution increased 32.4% under the optimum fermentation medium and culture conditions.The fermentative liquid of strain JM-3 could not cure canker lesion expansion,but it could prevent canker lesion expansion significantly.The preventive protection of strain JM-3 fermentative liquid was the best when it was inoculated on apple twigs before 24-48 hours,and its protective effect had no significant difference with difenoconazole.

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Last Update: 2018-02-24