|Table of Contents|

Screening of Antagonistic Actinomycete Against Strawberry Grey Mould and Activity Determination of Its Fermentation Broth

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年04
Page:
59-65
Research Field:
Publishing date:

Info

Title:
Screening of Antagonistic Actinomycete Against Strawberry Grey Mould and Activity Determination of Its Fermentation Broth
Author(s):
ZHAO Juan1; JIA Weiguo2; LIU Weicheng1; LIU Dewen1; ZHANG Dianpeng1; LU Caige1
1.Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097;2.Gu′an County Niutuo Town Garrison Production Base,Langfang,Hebei 065501
Keywords:
strawberry grey mould; Streptomyces tendae; inhibitory activity; culture filtrate stability
PACS:
-
DOI:
10.11937/bfyy.20172767
Abstract:
The actinomycetes obtained from Gurbantunggut desert in Xinjiang were used as test material to study their inhibitory effect on strawberry grey mould using agar block and mycelia growth inhibition methods.The actinomycete with high antagonistic activity was identified based on morphological features,physiological and biochemical characters,and 16S rDNA sequence analysis methods.The results showed that the antagonistic strain XJ93 with high activity was screened from 141 actinomycetes,the inhibition diameter against grey mould reached 20.67 mm.The strain also displayed obvious inhibitory activities against many plant fungal pathogens with inhibition rates 25.00%-71.67%.The culture filtrate of strain XJ93 showed good thermal stability,since the inhibitory rate was almost unchanged when the temperature rose to 80 ℃.The culture filtrate was also stable at pH 5-9,and maintained good inhibitory activity when exposed to a dose of light and ultraviolet irradiation for 150 minutes.The antagonistic actinomycete XJ93 was identified as Streptomyces tendae.It could be concluded that,S.tendae XJ93 had good inhibitory activity stability and broad inhibitory spectrum;therefore,it could potentially be used as biocontrol agent of plant fungal diseases including strawberry grey mould.

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Last Update: 2018-02-24