|Table of Contents|

Effect of Ammonium Stress on Cultured Blueberry Plantlets in vitro Based on FTIR Spectroscopy

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年04
Page:
48-53
Research Field:
Publishing date:

Info

Title:
Effect of Ammonium Stress on Cultured Blueberry Plantlets in vitro Based on FTIR Spectroscopy
Author(s):
TANG Ying; AN Lijia
College of Life Science and Biotechnology,Dalian University of Technology,Dalian,Liaoning 116024
Keywords:
Fourier transform infrared spectroscopy(FTIR); ammonium salt stress; calcium alleviation; in vitro cultured blueberry plantlets
PACS:
-
DOI:
10.11937/bfyy.20173056
Abstract:
In the current study,in vitro cultured blueberry plantlets were used as materials.The metabolites of in vitro cultured blueberry plantlets under normal growth,ammonium stress and calcium alleviation treatments were analyzed by Fourier transform infrared spectroscopy (FTIR),which provided further information for the mechanism of ammonium salt stress.The results demonstrated that FTIR peak shape and peak position were similar,and that chemical substances were substantially the same,including protein,lipid and carbohydrates and inorganic elements.Compared with the normal growth plants,lines under ammonium stress lacked the absorption peaks near 1 318 cm-1,which might correlate to the level of cell membrane lipids or the methylation of cell walls and cation exchange capacity.After CaCl2 application,lines showed an absorption peak near 668 cm-1compared with lines under ammonium stress,which might correlate to calcium absorption.Ammonium sulfate stress resulted in a decrease in carbohydrate,lipid and protein contents.The degradation of protein was much more than that of polysaccharides after stress.Supplemental Ca2+ could prevent ammonium damage and the accumulation of substances in blueberry plantlets,especially polysaccharides and proteins.

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Last Update: 2018-02-24