|Table of Contents|

Development of EST-SSR Markers and Optimization of SSR System in  Cerasus tianschanica(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年12
Page:
108-114
Research Field:
Publishing date:

Info

Title:
Development of EST-SSR Markers and Optimization of SSR System in  Cerasus tianschanica
Author(s):
QIN Xue1; CHEN Shuying2; TIAN Jia1; LI Peng1; SHENG Fang1; LI Jiang1
(1.College of Forestry and Horticulture,Xinjiang Agricultural University,Urumqi,Xinjiang 830052;2.Academy of Forestry in Ili of Xinjiang,Yining,Xinjiang 835000)
Keywords:
Cerasus tianschanica; primer development; EST-SSR; system optimization
PACS:
-
DOI:
10.11937/bfyy.201712026
Abstract:
Leaves of Cerasus tianschanica were used as materials.The EST sequences of Cerasus tianschanica were obtained from NCBI,SSR motifs were screened by using SSRHunter 1.3 software from these sequences,and specific primers were designed with the software Primer 6.0.Besides,the SSR-PCR reaction system of Cerasus tianschanica was optimized by orthogonal test and single factor experiment to verify the developed primers in order to lay the foundation for genetic diversity and construction of genetic map.The results showed that 21 pairs of primers could amplify specific bands,and the effective amplification rate was 70%.Moreover,the 20 μL reaction system contained 0.2 mmol?L-1 dNTPs,0.4 μmol?L-1 primer,0.75 U Taq polymerase and 60 ng template DNA.

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Last Update: 2017-06-27