|Table of Contents|

ISSR Analysis for Hybrid F1 of Trifolium ambiguum Bieb.and T.repens L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年12
Page:
103-107
Research Field:
Publishing date:

Info

Title:
ISSR Analysis for Hybrid F1 of Trifolium ambiguum Bieb.and T.repens L.
Author(s):
ZHOU Ying1; WANG Mingjiu1; CHEN Guoqing1; HUANG Fan2
(1.College of Grassland and Resource and Environmental Science,Inner Mongolia Agricultural University，Hohhot，Inner Mongolia 010019;2.Grassland Research Institute，Chinese Academy of Agricultural Sciences，Hohhot，Inner Mongolia 010010)
Keywords:
Trifolium ambiguum Bieb.;  T.repens L.; F1 hybrids; ISSR analysis
PACS:
-
DOI:
10.11937/bfyy.201712025
Abstract:
With Trifolium ambiguum Bieb.(2n=6×=48) as female parent and T.repens L.(2n=4×=32) as male parent，by outcrossing and embryo rescue technology，their hybrid F1 plants were obtained.Genetic difference degree of 9 strains hybrids F1 and their parents were analyzed via ISSR molecular markers.The results showed that 111 bands of ISSR markers were amplified with 16 primers，and length of DNA fragment ranged from 100 bp to 2 000 bp,72 bands were polymorphic，and percentage of polymorphic bands reached 64.86%.The genetic distance(GD) of 11 tested plant materials ranged from 0.073 4 to 0.477 1，with average of 0.241 2.The genetic distance among 9 strain hybrids F1 and T.repens L.was larger.The genetics similarity among 9 strain hybrids F1 and T.repens L.was less than Trifolium ambiguum Bieb.，9 strain hybrids F1 expressed more parental genetic information.

References:

 

[1]殷秀杰.高加索三叶草在内蒙古地区适应性和利用价值研究[D].呼和浩特:内蒙古农业大学,2004.

[2]魏春秋,王明玖.高加索三叶草青贮饲料的制作及质量变化[J].草业科学,2010(2):134-138.

[3]殷秀杰,王明玖,石凤翎.高加索三叶草和白三叶幼苗期抗旱性生理研究[J].内蒙古农业大学学报,2004,25(2):119-123.

[4]魏春秋.高加索三叶草生长特性及影响品质变化的因素[D].呼和浩特:内蒙古农业大学,2012.

[5]崔英.三种三叶草的耐盐性和抗寒性研究[D].呼和浩特:内蒙古农业大学,2006.

[6]ABBERTON M T，MACDUFF J H，MARSHALL A H，et al.Nitrogen fixation in hybrids of white clover(Trifolium repens L.)and Caucasian clover(Trifolium ambiguum M.Bieb.)[J].Blaekwell Wlssensehafts-veflag Gihoh,2000,105(4):241-247.

[7]杨珍.高加索三叶草与白三叶草及其杂交胚离体培养技术的研究[D].呼和浩特:内蒙古农业大学,2008.

[8]陈丽丽,王明玖,何丽君,等.高加索三叶草、白三叶及其杂种F1代的RAPD分析[J].中国草地学报,2011(5):40-44.

[9]栾晓敏.两种三叶草杂交胚离体培养与F1代扩繁及其鉴定[D].呼和浩特:内蒙古农业大学,2009.

[10]黄帆,王明玖,何丽君,等.高加索三叶草、白三叶及其杂种F1代的ISSR分析[J].中国草地学报,2012(6):107-112.

[11]黄帆.高加索三叶草与白三叶杂交后代遗传特性及育性恢复的研究[D].呼和浩特:内蒙古农业大学,2012.

[12]ZIETKIEWICZ E,RAFSLSKI A,LABUDA D.Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J].Genomics,1994(20):176-183

[13]黄帆,王明玖,何丽君,等.白三叶×高加索三叶草F1代茎段离体培养器官发生的研究[J].草业科学,2010(5):85-90.

[14]刘欢,慕平,赵桂琴,等.燕麦ISSR反应体系的建立与优化[J].中国草地学报,2010,32(4):80-85.

[15]王育青,王晓晶,王建光.马蔺ISSR-PCR反应体系的建立与优化[J].中国草地学报,2010,32(2):80-85.

[16]沙伟,藤兆岩,倪红伟.正交设计优化星星草ISSR-PCR反应体系研究[J].中国草地学报,2006,28(6):52-55.

[17]石凤玲,石凤翎,高翠萍,等.人工老化紫花苜蓿种子活力变化与ISSR标记[J].中国草地学报,2015(6):30-34.

[18]NEI M，LI W.Mathematical model for studying genetic variation in terms of restriction endonucleases[J].Proc Nat Acad Sci,1979，76:5269-5273.

[19]王瑜,袁庆华.紫花苜蓿ISSR-PCR反应体系的建立与优化[J].草地学报,2007,15(3):212-215.

[20]王方,袁庆华.冰草ISSR-PCR反应体系的建立与优化[J].草地学报,2009,17(3):354-357.

[21]刘青松,陈立波,李志勇,等.不同苜蓿农艺性状研究及 ISSR分子标记分析[J].华北农学报,2014,29(3):109-114.

[22]孟丽娟,赵桂琴.红三叶ISSR-PCR反应体系的建立与优化[J].草原与草坪,2015,35(2):21-25.

[23]索志立,张会金,张治明,等.紫斑牡丹与牡丹种间杂交后代的DNA分子证据[J].云南植物研究,2005(1):42-48.

[24]乔燕春,林顺权,何小龙.普通枇杷种内和种间杂种苗的RAPD鉴定[J].果树学报,2010(3):385-390.

[25]胡凤荣,何国仁,王斐,等.利用ISSR分子标记方法鉴定风信子杂种后代[J].分子植物育种,2015(6):1336-1342.

[26]秦泰辰.杂种优势利用原理和方法[M].南京:江苏科学技术出版社,1981.

[27]李竞雄,周洪生.作物雄性不育及杂种优势研究进展(I)[M].北京:中国农业出版社,1996.

[28]周亚星,于卓,马艳红,等.散穗高粱与苏丹草杂种的ISSR分析[J].草地学报,2009(6):718-722.

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Last Update: 2017-06-27