|Table of Contents|

Construction of Expression Vector Knocking Out ACC Synthase Gene in  Melon by CRISPR-Cas9 System(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年12
Page:
114-118
Research Field:
Publishing date:

Info

Title:
Construction of Expression Vector Knocking Out ACC Synthase Gene in  Melon by CRISPR-Cas9 System
Author(s):
WANG Xue; LI Guan
(College of Life Science and Technology，Xinjiang University，Urumqi，Xinjiang 830046)
Keywords:
CRISPR-Cas9; gene knockout; ACC synthase gene
PACS:
-
DOI:
10.11937/bfyy.201712027
Abstract:
The CRISPR-Cas9 genome editing techniques of melon，was constructed a vector which knockout ACC synthase gene by CRISPR-Cas9 system,and then the high efficient genome editing technique was obtained which could knockout melon genes.The results showed that based on the ACC synthase gene of melon to designed primers,and then they were builded to the pP1C.4 vector.The connect product was transformed into escherichia coli by heat shock method.Finally,a few positive clones were selected,they were final vector.

References:

 

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Last Update: 2017-06-27