|Table of Contents|

Establishment of Regeneration System for Cucumis melo L.cv.‘Jiashi’ of Xinjiang Melon(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年16
Page:
89-92
Research Field:
Publishing date:

Info

Title:
Establishment of Regeneration System for Cucumis melo L.cv.‘Jiashi’ of Xinjiang Melon
Author(s):
DU Shanshan; LI Siyi; WANG Dong; LI Guan
(College of Life and Technology，Xinjiang University，Urumqi，Xinjiang 830046)
Keywords:
melon; 6-BA; AgNO3; regeneration system
PACS:
-
DOI:
10.11937/bfyy.201616024
Abstract:
A high efficiency and rapid regeneration system of thick-skinned muskmelon had been set up using cultivars ‘Jiashi’ cultured in the media with different hormone of 6-Benzolaminopurine(6-BA) and silver nitrate(AgNO3).The 5 days sterile cotyledon of ‘Jiashi’ were cultured in media with different 6-BA concentration.The effect of different AgNO3 concentration added in media with the most appropriate 6-BA concentration was discussed.The results showed that the MSB medium containing 1.5 mg?L-1 6-BA was the most suitable for inducing adventitious buds with an average rate of 75%.The MSB medium containing 1.5 mg?L-1 6-BA+2.0 mg?L-1 AgNO3 was the best suitable for inducing adventitious buds with an average rate of 65%，there were most of single adventitious buds，this combination could save time of regeneration system.Adventitious buds were able to elongation on MSB medium with 0.1 mg?L-1 6-BA and 0.1 mg?L-1 GA3，adventitious buds elongated and grew well.Regenerated shoots were rooted on MSB medium with 0.01 mg?L-1 NAA.

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Last Update: 2016-09-18