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Cloning and Expression Analysis of Glyceraldehyde-3-phosphate Dehydrogenase From Ziziphus jujuba(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年13
Page:
102-106
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of Glyceraldehyde-3-phosphate Dehydrogenase From Ziziphus jujuba
Author(s):
BU Jiaodi1; CHEN Yingying2; LIU Mengjun2; ZHAO Jin1
1.College of Life Science，Agricultural University of Hebei，Baoding,Hebei 071000;2.Research Center of Chinese Jujube，Agricultural University of Hebei，Baoding，Hebei 071000
Keywords:
Ziziphus jujuba; glyceraldehyde-3-phosphate dehyrogenase; cloning; expression analysis
PACS:
Q 786;S 665
DOI:
10.11937/bfyy.201513030
Abstract:
Taking Ziziphus jujube Mill.‘Jinsixiaozao’as material,a fulllength cDNA sequence of GAPDH gene was cloned from Chinese jujube by homologous gene cloning method and RT-PCR method.The results showed that，the gene was 1 020 bp，the estimated molecular weight and isoelectric point of the putative protein were 36-904 kDa and 8.53，named as ZjGAPDH (accession number KP147910).The protein had two conserved domains named as Gp_dh_N superfamily and Gp_dh_C superfamily.Phylogenic analysis showed that ZjGAPDH had a low homology with that of other species but all belong to cytoplasmic proteins and involve in glycolysis.Qrt-PCR analysis showed that the ZjGAPDH expressions at different riping stages of jujube fruit were significantly different，and the expression level of ZjGAPDH at young fruit stage and whole-red ripening stage were higher than that of other stages.

References:

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Last Update: 2015-08-21