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Establishment of Populus deltoids cv.JuBa Regeneration System and Its Resistance to Hygromycin(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年13
Page:
98-102
Research Field:
Publishing date:

Info

Title:
Establishment of Populus deltoids cv.JuBa Regeneration System and Its Resistance to Hygromycin
Author(s):
WANG Guiying1; 2 ; LIU Xiaojie1; LI Shanshan2; SUN Chao1; YANG Jinku1; YANG Minsheng2; LIU Meiling1
1.Langfang Academy of Agriculture and Forestry Sciences,Langfang,Hebei 065000；2.Keylab of Genetic Resonrces of Forest and Forest Protection,Agricultural University of Hebei,Baoding,Hebei 071001
Keywords:
Populus deltoids cv.JuBa; regeneration system; hygromycin; critical concentration
PACS:
S 792.11
DOI:
10.11937/bfyy.201513029
Abstract:
Taking a hybrid poplar variety Populus deltoids cv.JuBa (Populus deltoids 50×P.deltoides 36) as experiment material，the effect of different phytohormone combination and hygromycin concentration gradient on leaves and shoot tips were studied.The results showed that the optimal medium for bud induction was MS+6 BA 0.25 mg/L+IBA 0.1 mg/L，the optimal regeneration and rooting media was 1/2MS+IBA 0.3 mg/L.Through screening different hygromycin concentrations for leaves and shoot tips，hygromycin could affect the growth of JuBa poplar.The hygromycin inhibitory effects increased with growth time.Leaves were more sensitive comparing to shoot tips.After 2 days of cultivation，leaves were obviously damaged.The damage of shoot tips began to show only after 4 days.After 2 weeks，only a few buds appeared on petiole on 4 mg/L hygromycin；all leaves and shoot tips were dead under above 6 mg/L of hygromycin.The results demonstrated that the range of hygromycin critical concentration was from 4 mg/L to 6 mg/L.This research laid a technological foundation for the genetic transformation of this poplar variety.

References:

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Last Update: 2015-08-21