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Study on Two Insect-resistant Gene Transformation of Poplar 107(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年07期
Page:
120-122
Research Field:
Publishing date:

Info

Title:
Study on Two Insect-resistant Gene Transformation of Poplar 107
Author(s):
YANG Yan-li; LIU Xing-ju; LIU Gui-lin; ZHOU Ning; FU Xiao-peng; YANG Xu; LIANG Hai-yong
Agricultural University of Hebei,Hebei Key Laboratory for Tree Genetic Resources and Forest Protection，Baoding,Hebei 071000
Keywords:
Poplar 107; Agrobacterium tumefaciens; genetic transformation; regeneration system; detection
PACS:
S 792.11
DOI:
-
Abstract:
Taking Poplar 107 as material,and two kinds of insect genes (BtCry1Ac genes and API genes) genetic transformation study was mediated by the agrobacterium,the impact factors to Poplar 107 during the genetic transformation were discussed,and the more efficient tissue culture regeneration and genetic transformation system for Poplar 107 were established.The results showed that after the PCR detection and worm test results,it proved that the aim gene had been integrated into the Popar 107 genome,and the target was to select the top-quality plants with normal growth and strongly construct insect ability from the transgenic plants.

References:

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Last Update: 2014-09-03