|Table of Contents|

Establish of High Frequency Regeneration System of Taraxacum kok-saghyz Rodin(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年07期
Page:
115-119
Research Field:
Publishing date:

Info

Title:
Establish of High Frequency Regeneration System of Taraxacum kok-saghyz Rodin
Author(s):
LUO Cheng-hua; YAN Jie; ZHU Jian-bo
College of Life Science,Shihezi University,Shihezi,Xinjiang 832003
Keywords:
Taraxacum kok-saghyz Rodin; regeneration system; rooting
PACS:
S 682.1+9
DOI:
-
Abstract:
The effects of disinfection time and exogenous hormones on the survival rate，induction of in vitro shoot，elongation and root formation were studied by taking young leaf of Taraxacum kok-saghyz Rodin as explants.The results showed that the optimum disinfection method was treated with 0.1% HgCl2 for 7 min；the optimum regeneration medium was MS+1.5 mg/L 6-BA+0.1 mg/L NAA and the average induction rate was 97.3%；the optimum shoot regeneration medium was MS+2.0 mg/L 6-BA+0.02 mg/L NAA+0.4 mg/L GA3；the best rooting medium was 1/2MS+0.2 mg/L NAA，the rooting rate was 100%，the tissue cultured plants obtained grew well and had high survival rate after transplanting，the optimum regeneration system of Taraxacum kok-saghyz Rodin was found finally.

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Last Update: 2014-09-03