QI Qingru,WANG Lei.Effects of the Concentration of Endophytic Mycorrhizal Fungi on the Growth Characteristics of Two Turfgrasses[J].Northern Horticulture,2024,(5):62-67.[doi:10.11937/bfyy.20233169]
内生菌根菌剂浓度对两种草坪草生长特性的影响
- Title:
- Effects of the Concentration of Endophytic Mycorrhizal Fungi on the Growth Characteristics of Two Turfgrasses
- 文章编号:
- 1001-0009(2024)05-0062-06
- Keywords:
- Poa praterrisis L.; Lolium perenne L.; endophytic mycorrhizal fungi agent; physiological and biochemical indexes
- 分类号:
- S 688.4
- 文献标志码:
- A
- 摘要:
- 以北方地区常用的多年生黑麦草和草地早熟禾为试材，施加不同浓度的内生菌剂，浓度梯度T1为10%，T2为30%，T3为50%，T0为不施加菌剂的空白对照，以菌剂拌种的方式施加菌剂，在正常养护60 d后做侵染检测，测量不同菌剂浓度下植物的根系活力、相对电导率、可溶性蛋白质、可溶性糖和叶绿素含量的指标变化，研究了施加不同浓度的菌剂对黑麦草和草地早熟禾的影响，以期筛选出最适菌剂浓度，提高植物的生长质量。结果表明：在所试植物材料中施加菌剂浓度在50%时侵染效果最佳，草地早熟禾和多年生黑麦草侵染率可达38.71%和46.73%，其中草地早熟禾根系活力提高至13.22%，电导率降低至14%，可溶性蛋白质含量达2.91 mg·g-1，可溶性糖含量达28.84 mg·g-1，叶绿素含量可达3.10 mg·g-1；而多年生黑麦草根系活力提高至114.42%，电导率降低至11.00%，可溶性蛋白质含量达3.51 mg·g-1，可溶性糖含量达27.28 mg·g-1，叶绿素含量可达3.22 mg·g-1。综上所述，施加50%浓度的菌剂可有效提升供试植物材料的生长质量。
- Abstract:
- Poa praterrisis L.and Lolium perenne L.commonly used in the northern region were used as plant experimental materials，and different concentrations of endophytic agents were applied.The concentration gradient was T1 of 10%，T2 of 30%，T3 of 50%，and T0 was the blank control group without the application of bacteria，which was used as the experimental treatment.The inoculum was applied by seed dressing，and the infection was detected after 60 days of normal maintenance.The root activity，relative conductivity，soluble protein，soluble sugar and chlorophyll content of plants under different inoculum concentrations were measured.The effects of different concentrations of microbial agents on P.praterrisis L.and L.perenne L.were studied，in order to screen out the optimal concentration of microbial agents and improve the growth quality of plants.The results showed that the infection effect was the best when the concentration of the agent was 50% in the tested plant materials.The infection rate of P.praterrisis L.and L.perenne L.could reach 38.71% and 46.73%,respectively.The root activity of P.praterrisis L.increased to 13.22%,the conductivity decreased to 14%,the soluble protein content reached 2.91 mg·g-1,the soluble sugar content reached 28.84 mg·g-1,and the chlorophyll content reached 3.10 mg·g-1.The root activity of L.perenne L.increased to 114.42%,the conductivity decreased to 11.00%,the soluble protein content reached 3.51 mg·g-1,the soluble sugar content reached 27.28 mg·g-1,and the chlorophyll content reached 3.22 mg·g-1.The comprehensive conclusion shows that the application of 50% concentration of bacterial agent could effectively improve the growth quality of the tested plant materials.
参考文献/References:
［1］赵金龙,唐芳林,刘永杰.生态文明建设背景下我国草坪业发展的思考［J］.草业科学,2021,38(10):2077-2086.［2］张美存,程田,多立安,等.微生物菌剂对草坪植物高羊茅生长与土壤酶活性的影响［J］.生态学报,2017,37(14):4763-4769.［3］吴俊仪.呼和浩特市草地早熟禾春季萌动机制的研究［D］.呼和浩特:内蒙古农业大学,2018.［4］刘佳星.呼和浩特大青山草地早熟禾与新歌来德品种竞争特性研究［D］.呼和浩特:内蒙古农业大学,2019.［5］张露露,刘兴菊,马源,等.7个常用草地早熟禾品种对立枯丝核菌侵染的生理响应［J］.草原与草坪,2022,42(3):81-90.［6］张子嘉.不同微生物菌剂对多年生黑麦草耐热性与抗旱性的影响［D］.荆州:长江大学,2022.［7］韩玉竹,曾兵,黄建国.多花黑麦草根际微生物研究［J］.中国草地学报,2011,33(4):78-82.［8］陈丽娜,刘晓华,曹荷艳，等.丛枝菌根真菌在园艺植物中的应用［C］//张启翔，等.中国观赏园艺研究进展2012.北京：中国林业出版社,2012.［9］夏建国,李静.利用丛枝菌根真菌(AMF)提高植物抗旱性的研究进展［J］.中国农学通报,2005(2):326-329.［10］LIU R，YANG L，ZOU Y，et al.Root-associated endophytic fungi modulate endogenous auxin and cytokinin levels to improve plant biomass and root morphology of trifoliate orange［J］.Horticultural Plant Journal,2023,9(3):463-472.［11］PONS S,FOURNIER S,CHERVIN C,et al.Phytohormone production by the arbuscular mycorrhizal fungus Rhizophagus irregularis［J］.PLoS One,2020,15(10):e0240886.［12］许平辉.丛枝菌根真菌（AMF）对水分胁迫下茶树生长及抗旱性的影响［D］.杨凌:西北农林科技大学,2017.［13］柴晓虹,李录山,姚拓,等.浅谈微生物肥料的作用效果［J］.四川农业科技,2020(2):49-52.［14］韩玮.丛枝菌根真菌对镉胁迫下黑麦草光合生理的响应［J］.中国土壤与肥料,2022(6):204-212,220.［15］王国斌.内生真菌对多年生黑麦草耐重金属胁迫的影响［D］.兰州:兰州大学,2020.［16］刘丽丽,李建辉,陈骏,等.丛枝菌根真菌在果树研究中的应用［J］.浙江柑橘,2021,38(2):9-13.［17］郝志鹏,谢伟,陈保冬.丛枝菌根真菌在农业中的应用:研究进展与挑战［J］.科技导报,2022,40(3):87-98.［18］CHEN Y，HUI W.New microbiology and biotechnology study findings reported frominstitute of technology (Arbuscular mycorrhizal fungi in phytoremediation of contaminated areas by trace elements：Mechanisms and major benefits of their applications)［J］.Biotech Week,2015,14(2):322-322.［19］杨慧娟.丛枝菌根真菌在内蒙古草原区铁尾矿废弃地植被恢复中的作用研究［D］.呼和浩特:内蒙古大学,2012.［20］阚海明,徐恒康,鲁佳男,等.不同丛枝菌根真菌接种对3种草地植物生长特性的影响［J］.草地学报,2023,31(7):1922-1930.［21］张子嘉,肖波.微生物菌剂对草坪草的促生机理及增强抗逆性研究进展［J］.草学,2022(2):5-8.［22］王兰梦,郑立昊,郎多勇等.微生物肥料及其在中药生态农业中的前景分析［J］.北方园艺,2022(2):141-147.
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备注/Memo
第一作者简介：齐庆茹（1995-），女，硕士研究生，研究方向为风景园林与人居环境。E-mail:qiqingru@foxmail.com.责任作者：王雷（1972-），男，博士，教授，现主要从事草坪生态学与园林植物等研究工作。E-mail:14747405563@163.com.基金项目：内蒙古自治区应用技术研究与开发资金资助项目（2021GG0075）；乌海市全域矿山排土场植被生态恢复规划资助项目（WHZCGF230038）。收稿日期：2023-09-14