ZHANG Xiangyu,CHEN Xiaofang,LIU Min,et al.Diversity of Arbuscular Mycorrhizal Fungi in Different Habitats of Paris polyphylla and Their Correlation With the Environment[J].Northern Horticulture,2024,(6):69-78.[doi:10.11937/bfyy.20232879]
不同生境滇重楼丛枝菌根真菌多样性与环境相关性
- Title:
- Diversity of Arbuscular Mycorrhizal Fungi in Different Habitats of Paris polyphylla and Their Correlation With the Environment
- 文章编号:
- 1001-0009(2024)06-0069-10
- 分类号:
- S 151.9+4
- 文献标志码:
- A
- 摘要:
- 以不同生境重楼根际土壤、根部组织为试材，采用高通量测序和生物信息学方法，研究不同生境滇重楼AMF群落组成，并对土壤环境因子进行相关性分析，以期为重楼专用菌剂研发、提高栽培效益提供参考依据。结果表明：NMDS分析和样本层级聚类均只有WNT相对独立，其他样本均有交织；根部AMF多样性大小为ZJG>WNG（DFG）、根际土壤为ZJT>WNT>DFT，共有OTU为0；丰度前11的种均与ACP、OM、SC呈显著正相关，与TP、AP、AK、PPO、TK、pH、URE呈显著负相关；呈正相关性的菌均分布于ZJ样本，关系更为密切；通过NCBI序列比对得到缩隔球囊霉、幼套近明球囊霉、巴西无梗囊霉、摩西管柄囊霉、盾孢囊霉、柯氏无梗囊霉等菌种。AMF的聚集性受地理距离和局域环境因子交织影响，菌群结构受海拔、生境、土壤理化因子的共同作用，巴西无梗囊霉、摩西管柄囊霉等菌种对滇重楼生长发育具有重要意义，可利用这些菌种开发生物菌剂提高滇重楼栽培效益。
- Abstract:
- Taking the rhizosphere soil and root tissue of Paris polyphylla in different habitats as test materials，high-throughput sequencing and bioinformatics methods were used to study the composition of the AMF community of P.polyphylla in different habitats，and correlation analysis was conducted on soil environmental factors,in order to provide reference for the development of specialized bacterial agents for P.polyphylla and the improvement of cultivation efficiency.The results showed that both NMDS analysis and sample level clustering showed that only WNT was relatively independent，while other samples were interleaved.The diversity of root AMF was ZJG>WNG (DFG)，rhizosphere soil was ZJT>WNT>DFT，and the total OTU was 0.The top 11 species with abundance showed a significant positive correlation with ACP，OM，SC，and a significant negative correlation with TP，AP，AK，PPO，TK，pH，URE；The bacteria with positive correlation were all distributed in ZJ samples，and the relationship was more closely related.Through NCBI sequence alignment，strains such as Septoglomus constrictum,Claroideoglomus etunicatum,Acaulospora brasiliensis,Funneliformis mosseae,Scutellospora calospora，and Acaulospora koskei were obtained.The aggregation of AMF was influenced by the interweaving of geographical distance and local environmental factors.The microbial community structure was influenced by the combined effects of altitude，habitat，and soil physicochemical factors.Fungi such as Acaulospora brasiliensis and Funneliformis mosseae were of great significance for the growth and development of P.polyphylla.These strains could be used to develop biological agents to improve the cultivation efficiency of P.polyphylla.
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备注/Memo
第一作者简介：张翔宇（1986-），男，硕士，研究员，现主要从事中草药种植等研究工作。E-mail：304626335@qq.com.基金项目：贵州省科技计划资助项目（黔科合支撑［2020］4Y110号）；贵州省人才基地资助项目(RCJD2020-21)；贵州省高层次创新型人才资助项目（毕科人才合字［2021］05号）；广州市科技计划资助项目（2023B03J1292）。收稿日期：2023-08-21