QIU Yanqiu,CHEN Jiaying,YANG Linli,et al.Transcriptome Analysis of Chrysanthemum morifolium Under Drought Stress[J].Northern Horticulture,2025,(3):9-17.[doi:10.11937/bfyy.20242097]
干旱胁迫下药菊转录组分析
- Title:
- Transcriptome Analysis of Chrysanthemum morifolium Under Drought Stress
- 文章编号:
- 1001-0009(2025)03-0009-09
- Keywords:
- Chrysanthemum morifolium (Ramat); drought stress; transcriptome; differentially expressed genes; metabolic pathways
- 分类号:
- S 682.1+1
- 文献标志码:
- A
- 摘要:
- 以“亳菊”和“杭菊”为试材，采用高通量转录组测序方法，研究了干旱胁迫下盛花期“亳菊”和“杭菊”差异表达基因变化和代谢通路变化情况，以期为明确药菊应对干旱胁迫的分子机制提供参考依据。结果表明：差异表达基因（differentially expressed genes）筛选结果发现，干旱胁迫下“亳菊”和“杭菊”分别筛选出28 145、4 831个DEGs，“亳菊”富集到的DEGs数目更多。KEGG代谢途径富集分析发现，干旱胁迫下“亳菊”中的DEGs主要富集在氧化磷酸化、氨基酸和核苷酸糖代谢、半乳糖代谢、半胱氨酸和甲硫氨酸代谢、苯丙烷类生物合成、二苯乙烯、二芳基庚烷和姜酚的生物合成、类黄酮生物合成等，“杭菊”的DEGs主要富集在光合作用-触角蛋白、丙氨酸、天冬氨酸和谷氨酸代谢、苯丙烷类生物合成、二苯乙烯、二芳基庚烷和姜酚的生物合成、类黄酮生物合成。通过转录组测序，初步筛选了“亳菊”和“杭菊”干旱处理后的差异表达基因，揭示了“亳菊”主要通过增强能量代谢、氨基酸代谢、次生代谢来响应干旱胁迫，“杭菊”主要通过增强碳水化合物代谢、氨基酸代谢、次生代谢、光合作用来响应干旱。
- Abstract:
- Taking C.morifolium ‘Boju’ and C.morifolium ‘Hangju’ as the test materials，the changes of differentially expressed genes and metabolic pathways in C.morifolium ‘Boju’ and C.morifolium ‘Hangju’ during the blooming period under drought stress were investigated by using high-throughput transcriptome sequencing，in order to provide a reference for clarifying the molecular mechanisms of C.morifolium in response to drought stress.The results showed that 28 145 and 4 831 DEGs were identified in C.morifolium ‘Boju’ and C.morifolium ‘Hangju’，respectively，under drought stress，with more DEGs enriched in C.morifolium ‘Boju’.The KEGG metabolic pathway enrichment analysis revealed that the DEGs in C.morifolium ‘Boju’ were mainly enriched in the areas of oxidative phosphorylation，amino acid and nucleotide sugar metabolism，galactose metabolism，cysteine and methionine metabolism，phenylpropanoid biosynthesis，stilbenoid，diarylheptanoid and gingerol biosynthesisand flavonoid biosynthesis，etc.，whereas in C.morifolium ‘Hangju’，DEGs were mainly concentrated in photosynthesis-antenna proteins，alanine，aspartate and glutamate metabolism，phenylpropanoid biosynthesis，stilbenoid，diarylheptanoid and gingerol biosynthesisand flavonoid biosynthesis，etc.Preliminary screening of differentially expressed genes after drought treatment in C.morifolium ‘Boju’ and C.morifolium‘Hangju’ by transcriptome sequencing revealed that C.morifolium ‘Boju’ responded to drought stress mainly by enhancing energy metabolism，amino acid metabolism，and secondary metabolism，while C.morifolium ‘Hangju’ responded to drought mainly by enhancing carbohydrate metabolism，amino acid metabolism，secondary metabolism，and photosynthesis.
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备注/Memo
第一作者简介：邱艳秋（1997-），女，硕士研究生，研究方向为药用植物栽培。E-mail：hnautcm@126.com.责任作者：张红瑞（1978-），女，博士，教授，现主要从事中药资源与栽培等研究工作。E-mail：zhanghongrui2003@126.com.基金项目：河南省高等学校重点科研资助项目（19A210005,23A210002）；河南省中药材产业科技特派员服务团资助项目（2024）；河南省财政科技兴林资助项目（YLK202305）。收稿日期：2024-05-19