JIN Xin,ZHANG Jinpeng,ZHAO Yanfei,et al.Molecular Marker Screening of Germplasm Resources for Watermelon Flesh Color and Other Traits[J].Northern Horticulture,2024,(21):30-36.[doi:10.11937/bfyy.20241743]
西瓜果肉颜色等性状种质资源分子标记筛选
- Title:
- Molecular Marker Screening of Germplasm Resources for Watermelon Flesh Color and Other Traits
- 文章编号:
- 1001-0009(2024)21-0030-07
- Keywords:
- watermelon; molecular marker; flesh color; flesh hardness; resistance to blight
- 分类号:
- S 651
- 文献标志码:
- A
- 摘要:
- 以110份西瓜种质资源为试材,采用分子标记技术,研究了不同试验材料的果肉颜色、果肉硬度及枯萎病抗性的差异,以期为后续的新品种选育工作提供参考依据。结果表明:筛选出兼具硬肉和高抗枯萎病种质资源6份;兼具非硬肉和高抗枯萎病种质资源23份;兼具红肉和高抗枯萎病种质资源16份;兼具粉红肉和高抗枯萎病种质资源4份。未筛选到兼具红肉、硬肉和高抗枯萎病的种质。
- Abstract:
- Taking 110 watermelon germplasm resources as the test materials and adopted molecular marker technology to investigate the differences in flesh color,flesh firmness and resistance to blight of different test materials,in order to provide reference for the subsequent breeding of new varieties.The results showed that 6 germplasm resources with both hard meat and high resistance to blight were selected.There were 23 germplasm resources with both non-hard meat and high resistance to blight.16 germplasm resources with both red meat and high resistance to blight;both pink meat and high resistance to blight germplasm resources 4.No germplasm with both red meat,hard meat and high resistance to blight was screened.
参考文献/References:
[1]赵姜.中国西瓜产业发展的经济学分析[D].北京:中国农业科学院,2013.[2]SONG H,LEE K,SUBBURAJ S,et al.〖STBX〗ClPSY1 〖STBZ〗gene-based SNP markers identified from whole-genome resequencing for the determination of orange flesh color and carotenoid content in watermelon[J].Scientia Horticulturae,2023,318:112120.[3]姬万丽,袁平丽,赵胜杰,等.抗枯萎病和白粉病西瓜种质的分子标记筛选研究[J].分子植物育种,2017,15(7):2702-2710.[4]张金鹏,金鑫,赵艳菲,等.西瓜分子标记辅助育种研究与应用进展[J].中国瓜菜,2022,35(4):1-7.[5]栾非时,刘识,高鹏,等.鉴定西瓜果肉颜色的方法,引物对,试剂盒及其应用[P].中国:CN113151563A,2024-08-21.[6]许勇,张洁,宫国义,等.用于判断西瓜果肉硬度的SNP位点,Hf-KASP1标记及其应用[P].中国:CN202011446976.3,2024-08-21.[7]刘广,黄晓云,徐锦华,等.西瓜枯萎病抗性分子标记筛选与应用[J].江苏农业科学,2022,50(18):279-283.[8]DOU J,WANG Y,YANG H,et al.Development of branchless watermelon near isogenic lines by marker assisted selection[J].Horticultural Plant Journal,2022,8(5):627-636.[9]许勇,张洁,宫国义,等.用于判断西瓜果肉硬度的SNP位点,Hf-KASP1标记及其应用[P].中国:CN202011446976.3,2024-08-21.[10]许勇,张洁,张海英,等.一种与西瓜果肉硬度相关的分子标记Hf2-Indel及其应用[P].中国:CN106011284B,2019-10-11.[11]李娜,尚建立,马双武,等.鉴定西瓜枯萎病的InDel分子标记及其引物和应用[P].中国:CN105331615B,2017-12-01.[12]栾非时,闫令文,刘树森,等.西瓜种质资源背景选择标记筛选及遗传多样性分析[J].东北农业大学学报,2021,52(7):19-32.[13]张磊,武竞春,张海英,等.西瓜重要性状功能基因研究进展[J].园艺学报,2023,50(12):2748-2764.[14]刘广,黄晓云,徐锦华,等.西瓜枯萎病抗性分子标记筛选与应用[J].江苏农业科学,2022,50(18):279-283.[15]ZHANG J,SUN H,GUO S,et al.Decreased protein abundance of lycopene β-cyclase contributes to red flesh in domesticated watermelon[J].Plant Physiology,2020,183(3):1171-1183.[16]李圣龙.西瓜橙色果肉关键基因Clorf的精细定位[D].哈尔滨:东北农业大学,2023.[17]SUN L,ZHANG Y,CUI H,et al.Linkage mapping and comparative transcriptome analysis of firmness in watermelon (Citrullus lanatus)[J].Frontiers in Plant Science,2020(11):831.[18]孙蕾.西瓜中心果肉硬度主效QTL及候选基因分析[D].哈尔滨:东北农业大学,2021.[19]MA K,KOU J,KHASHI U RAHMAN M,et al.Palmitic acid mediated change of rhizosphere and alleviation of Fusarium wilt disease in watermelon[J].Saudi Journal of Biological Sciences,2021,28(6):3616-3623.[20]易丽聪,王运强,龚钰,等.西瓜种质抗病性的分子标记检测和人工接种鉴定[J].中国瓜菜,2021,34(9):7-14.[21]REN Y,SUN H,ZONG M,et al.Localization shift of a sugar transporter contributes to phloem unloading in sweet watermelons[J].New Phytologist,2020,227(6):1858-1871.[22]REN Y,LI M,GUO S,et al.Evolutionary gain of oligosaccharide hydrolysis and sugar transport enhanced carbohydrate partitioning in sweet watermelon fruits[J].The Plant Cell,2021,33(5):1554-1573.[23]段雅如,高美玲,郭宇,等.西瓜果形基因图位克隆及分子标记开发[J].中国农业科学,2022,55(14):2812-2824.[24]李娜,尚建立,李楠楠,等.西瓜果实形状的分子精准鉴定[J].园艺学报,2021,48(7):1386-1396.[25]MANDAL M K,SUREN H,KOUSIK C.Elucidation of resistance signaling and identification of powdery mildew resistant mapping loci (ClaPMR2) during watermelon-Podosphaera xanthii interaction using RNA-Seq and whole-genome resequencing approach[J].Scientific Reports,2020,10(1):14038.[26]张敬敬,张海英,潘秀清,等.河北省130份西瓜品种与种质资源抗病基因KASP检测分析[J].华北农学报,2019,34(2):110-116.
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备注/Memo
第一作者简介:金鑫(1992-),男,硕士,助理研究员,现主要从事西瓜育种和栽培等研究工作。E-mail:952143825@qq.com.责任作者:惠长敏(1965-),女,本科,研究员,现主要从事西甜瓜新品种选育等研究工作。E-mail:huichm@126.com.基金项目:吉林省科技发展计划资助项目(20210202087N C);国家西甜瓜产业技术体系建设专项资助项目(CARS-25)。收稿日期:2024-04-23