YANG Jianjie,ZHANG Guixiang,LIU Mingjun,et al.Study on Formula Screening and Extracellular Enzyme Activity of Lentinula edodes Cultivated by Corn Straw[J].Northern Horticulture,2024,(24):98-104.[doi:10.11937/bfyy.20241403]
玉米秸秆栽培香菇的配方筛选及胞外酶活性研究
- Title:
- Study on Formula Screening and Extracellular Enzyme Activity of Lentinula edodes Cultivated by Corn Straw
- 文章编号:
- 1001-0009(2024)24-0098-07
- 分类号:
- S 646.1+2
- 文献标志码:
- A
- 摘要:
- 以玉米秸秆和木屑为试材，采用单因素随机区组试验设计，设置6个不同比例的玉米秸秆与木屑配方，研究了不同配方基质对香菇菌丝生长速度、胞外酶活性、子实体形态、生物学效率和投入产出比的影响，以期为香菇草腐化栽培提供参考依据。结果表明：随着培养料中玉米秸秆含量的增加，培养料单位体积的干物质质量和碳氮比逐渐降低，菌丝生长逐渐变得细弱缓慢，胞外酶活性、生物学效率和投入产出比呈现出先升高后降低的趋势。综上可知，玉米秸秆栽培香菇的添加量不易超过48%。玉米秸秆添加量为16%时（木屑64%、玉米秸秆16%、麸皮18%、石膏粉1%、蔗糖1%），生物学效率和投入产出比最高，分别达到77.30%和1∶1.44。
- Abstract:
- Taking corn straw and sawdust as the test materials，six different proportions of corn straw and sawdust formulas were set up by single factor randomized block experimental design.The effects of different formula substrates on the mycelial growth rate，extracellular enzyme activity，fruiting body morphology，biological efficiency and input-output ratio of Lentinula edodes were studied，in order to provide reference for the decay cultivation of Lentinula edodes.The results showed that with the increase of corn straw content in the substrate，the dry matter mass and carbon-nitrogen ratio per unit volume of the substrate gradually decreased，the mycelial growth gradually became weak and slow，and the extracellular enzyme activity，biological efficiency and input-output ratio showed a trend of increasing first and then decreasing.In conclusion,the addition of corn straw to cultivate Lentinula edodes was not likely to exceed 48%.When the addition amount of corn straw was 16% (sawdust 64%，corn straw 16%，bran 18%，gypsum powder 1%，sucrose1%)，the biological efficiency and input-output ratio were the highest，reaching 77.30% and 1∶1.44，respectively.
参考文献/References:
［1］谭琦.中国香菇产业发展［M］.北京:中国农业出版社,2017.［2］刘国宇,马晓颖,李超,等.基于玉米秸秆基料的滑菇栽培基质配方筛选试验［J］.农业科技与装备,2022(3):7-9,12.［3］樊廷录,吕迎春,杨天育,等.甘肃省粮食生产分析及产能提升战略构思［J］.干旱地区农业研究,2023,41(4):1-8.［4］张金霞,蔡为明,黄晨阳.中国食用菌栽培学［M］.北京:中国农业出版社,2020.［5］聂倾国,聂永芳.玉米秸秆的饲料化利用及其对饲养成本和经济效益的影响［J］.中国饲料,2023(24):118-120.［6］黄年来,林志彬,陈国良,等.中国食药用菌学［M］.上海:上海科学技术文献出版社,2010.［7］杨建杰,张桂香,杨琴,等.不同原料基质栽培平菇的生物学研究［J］.西北林学院学报,2016,31(3):170-174.［8］张春艳,魏雅冬,胡畔等,杨木屑与玉米秸秆配方基质对平菇生长、营养价值及产量的影响［J］.北方园艺,2023(20):117-125.［9］赵红霞,詹勇,杨建军.白腐真菌降解秸秆作物研究及应用［J］.中国饲料,2003(15):25-27.［10］信召哲,赵庆新,唐伯平.香菇转化利用秸秆进程中多聚物降解酶作用研究现状［J］.中国食用菌,2016,35(6):10-13.［11］聂志强.产纤维素酶真菌的筛选及其酶学性质的研究［J］.中国酿造,2009,28(3):81-83.［12］ZHANG G M,HUANG J,HUANG G R,et al.Molecular cloning and heterologous expression of a new xylanase gene from Plectosphaerella cucumerina［J］.Applied Microbiology and Biotechnology,2007,74(2):339-346.［13］陈黄曌,邓晓旭,霍婧媛.玉米秸秆的再利用:代料栽培秋季香菇初探［J］.资源节约与环保,2017(11):103-104.［14］罗霄天,李森,秦心儿,等.高粱秸秆的设施化代料栽培对香菇产量和品质的影响［J］.生物资源,2024,46(1):11-19.［15］赖腾强,吴伯文,谢娜,等.利用枇杷枝屑代料栽培香菇技术［J］.现代园艺,2018(17):75-76.［16］胡润芳,张玉梅,陈伟,等.大豆秸秆代料栽培香菇试验［J］.东南园艺,2017,5(2):13-15.［17］张清凤,刘元剑,杨静康,等.苹果枝木屑作栽培料生产香菇效果试验［J］.上海蔬菜,2022(5):73-74.［18］夏建红,汪宏亮,郭菊梅,等.北方地区香菇棚栽越夏出菇技术［J］.北方园艺,2022(13):152-156.［19］李志刚,陈霞娜,丁李春,等.适宜葡萄枝屑栽培的香菇菌株筛选［J］.食用菌,2022,44(1):21-23.［20］楚晓真,崔杏春,刘格,等.不同配方培养料对香菇胞外酶活性、产量及品质的影响［J］.中国瓜菜,2023,36(3):110-114.［21］胡宝,杨诺林,崔嫚,等.香菇液体菌种培养过程中几个生理指标的变化规律研究［J］.食药用菌,2022,30(6):428-431.［22］管婉.香菇菌丝后熟转色的生理特征分析及应用［D］.扬州:江苏海洋大学,2022.［23］张建,黄天宇,鲍大鹏,等.利用转录组分析光照对香菇菌丝生长的影响［J］.食用菌学报,2023,30(3):1-10.［24］管婉,储婷,鲍大鹏,等.香菇菌丝转色过程中生理指标检测与分析［J］.食用菌学报,2021,28(6):47-52.［25］熊雪,李鹏,王莹,等.不同栽培辅料诱导对3个香菇菌株液体发酵产漆酶活力的影响［J］.江苏农业科学,2021,49(19):172-179.
相似文献/References:
[1]郭 丽,王 鹏,马志婷.香菇固态发酵对蓝莓果渣产物活性成分的研究[J].北方园艺,2014,38(11):143.
GUO Li,WANG Peng,MA Zhi-ting.Study on Active Ingredient of Blueberry Pomace in Solid-state Fermentation by Lentinus edodes [J].Northern Horticulture,2014,38(24):143.
[2]徐彦军,吴秀泉,任志华,等.贵州山区香菇菌株栽培比较试验[J].北方园艺,2013,37(06):153.
XU Yan-jun,WU Xiu-quan,REN Zhi-hua,et al.Cultivation Experiment of Lentinus edodes Strains in Guizhou Mountainous Area[J].Northern Horticulture,2013,37(24):153.
[3]童静,侯建伟.玉米秸秆型基质对唐菖蒲子球生理生化指标的影响[J].北方园艺,2012,36(04):61.
TONG Jing,HOU Jian-wei.The Effect of Several Index of Physiological and Biochemical Characters for Corn Straw Substrates on Gladiolus Cormels[J].Northern Horticulture,2012,36(24):61.
[4]李盛旻,王永宏,王广耀.香菇山楂复合果丹皮的研制[J].北方园艺,2012,36(21):126.
LI Sheng-min,WANG Yong-hong,WANG Guang-yao.Development of Mushrooms the Hawthorn Composite Sweetend Roll[J].Northern Horticulture,2012,36(24):126.
[5]贺伟强,巢新冬,白杰.香菇中多酚类物质提取工艺的优化研究[J].北方园艺,2013,37(18):125.
HEWei-qiang,CHAOXin-dong,BAIJie.StudyontheOptimizationoftheExtractionTechnologyof TotalPhenolicfromLentinulaedodes[J].Northern Horticulture,2013,37(24):125.
[6]赵志顺,逄振茹.辽宁东部山区人工栽培香菇过程中畸形菇发生原因及预防[J].北方园艺,2013,37(18):143.
ZHAOZhi-shun,PANGZhen-ru.CausesandPreventionofDeformedFruitingBodyintheProcessofArtificialCultavitionof LentinusedodesintheMountainousAreasofEasternLiaoning[J].Northern Horticulture,2013,37(24):143.
[7]赵娜娜,陈文强,邓百万,等.秦巴山区香菇主要栽培种亲缘关系的研究[J].北方园艺,2014,38(16):140.
ZHAO Na-na,CHEN Wen-qiang,DENG Bai-wan,et al.Study of the Genetic Relationships of Main Lentinula edodes Cultispecies in Qinba Mountainous Area[J].Northern Horticulture,2014,38(24):140.
[8]翁梁,张科,陈赟,等.玉米秸秆与菌糠栽培真姬菇比较研究[J].北方园艺,2014,38(17):143.
WENG Liang,ZHANG Ke,CHEN Yun,et al.Comparative Study on Cultivation of Hypsizygus marmoreus with Corn Stover and Spent Mushroom Substrate[J].Northern Horticulture,2014,38(24):143.
[9]宋莹,刘娜,肖千明,等.十五个香菇菌株遗传特异性研究[J].北方园艺,2014,38(18):163.
SONG Ying,LIU Na,XIAO Qian-ming,et al.Study on Genetic Specificity of 15 Lentinula edodes Strains[J].Northern Horticulture,2014,38(24):163.
[10]阙斐,冯文婕.提取工艺对丹参、五味子、香菇提取物抗氧化活性影响研究[J].北方园艺,2014,38(18):167.
QUE Fei,FENG Wen-jie.Effect of Extraction Technology on the Antioxidant Activity of Salviae miltiorrhizae Radix，Schisandra chinensis Fructus and Lentinula edodes[J].Northern Horticulture,2014,38(24):167.
备注/Memo
第一作者简介：杨建杰（1977-），男，本科，副研究员，现主要从事食用菌栽培技术研究与示范推广等工作。E-mail：yangjj0519@gsagr.cn.责任作者：张桂香（1964-），女，本科，研究员，现主要从事食用菌栽培技术研究与示范推广等工作。E-mail：zhanggx2008@sina.com.基金项目：甘肃省科技计划资助项目（22CX2NA001）；国家食用菌产业技术体系资助项目（CARS-20）；甘肃省现代农业科技支撑体系区域创新中心重点科技资助项目（2021GAAS56）。收稿日期：2024-04-03