[1]孟 丽,张兰增,周和云,等.数字基因表达谱解析草菇子实体的生长发育[J].北方园艺,2018,42(08):1-6.[doi:10.11937/bfyy.20173248]
　MENG Li,ZHANG Lanzeng,ZHOU Heyun,et al.Digital Gene Expression Profiling Reveals Volvariella volvacea Fruiting Body Development[J].Northern Horticulture,2018,42(08):1-6.[doi:10.11937/bfyy.20173248]
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数字基因表达谱解析草菇子实体的生长发育

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年08 页码: 1-6 栏目: 出版日期: 2018-04-30
Title:
Digital Gene Expression Profiling Reveals Volvariella volvacea Fruiting Body Development
作者:
孟 丽1; 张兰增1; 周和云1; 周森1; 李 玉2; 谢宝贵3
1.山东农业大学 植物保护学院，山东 泰安 271018；2.吉林农业大学 食药用菌教育部工程研究中心，吉林 长春 130118；3.福建农林大学 生命科学学院菌物研究中心，福建 福州 350002
Author(s):
MENG Li1;  ZHANG Lanzeng1;  ZHOU Heyun1;  ZHOU Sen1;  LI Yu2;  XIE Baogui3
1.College of Plant Protection, Shandong Agricultural University, Tai’an,Shandong 271018；2.Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun,Jilin 130118；3.Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002
关键词:
草菇; 数字基因表达谱; 子实体发育; 转录因子
Keywords:
Volvariella volvacea;  digital gene expression profiling;  fruiting body development;  transcription factor
DOI:
10.11937/bfyy.20173248
文献标志码:
A
摘要:
为筛选出与草菇子实体发育相关的转录因子，以不同发育时期的草菇子实体为材料，利用数字基因表达谱对不同发育时期的基因进行表达分析。结果表明：获得8个差异表达的Homeobox转录因子，其中5个在草菇原基期阶段上调表达，3个在伸长期阶段下调表达。这些差异表达的转录因子可能参与草菇子实体不同发育阶段的形态建成。
Abstract:
In order to select the transcription factors involved in fruiting body development in Volvariella volvacea, taking fruiting body in different development periods as test material, the gene expression was analyzed by digital gene expression. The results showed that eight differentially expressed homeobox transcription factors were obtained, including five with up-regulated expression in primordia and three with down-regulated expression in the elongation stage of V. volvacea. These differentially expressed homeobox transcription factors may contribute to morphological variation in V. volvacea with respect to fruiting body development.

参考文献/References:

[1] LANDER ES,LINTON LM,BIRREN B,et al.Initial sequencing and analysis of the human genome [J].Nature,2001,409(6822):860-920.

[2] GOLDMAN B,BHAT S,SHIMKETS L J.Genome Evolution and the Emergence of Fruiting Body Development in Myxococcus xanthus [J].Plos One,2007,2(12):e1329.
[3] CHRISTENSEN K,MURRAY JC.What genome-wide association studies can do for medicine [J].New England Journal of Medicine,2007,356(11):1094-1097.
[4] MENG L,YAN JJ,XIE BG,et al.Genes encoding FAD-binding proteins in Volvariella volvacea exhibit differential expression in homokaryons and heterokaryons [J].Microbiological Research,2013,168: 533-546.
[5] 王海英.草菇不同发育时期菌柄基因表达谱差异初步分析[D].福州:福建农林大学,2011.
[6] 鄢淑琴.采后不同时期的草菇菌柄表达谱差异分析[D].福州:福建农林大学,2011.
[7] MORRISSY AS,MORIN RD,DELANEY A,et al.Next-generation tag sequencing for cancer gene expression profiling [J].Genome Research,2009,19:1825-1835.
[8] AC’T HOEN P,ARIYUREK Y,THYGESEN HH,et al.Deep sequencing-based expression analysis shows major advances in robustness,resolution and inter-lab portability over five microarray platforms[J].Nucleic Acids Research.2008.36:e141.
[9] AUDIC S,CLAVERIE JM.The significance of digital gene expression profiles[J].Genome Research,1997,7:986-995.
[10] BENJAMINI Y,YEKUTIELI D.The control of the false discovery rate in multiple testing under dependency[J].Annals of Statistics,2001,1165-1188.
[11] THOMPSON JD,HIGGINS DG,GIBSON TJ.CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice[J].Nucleic Acids Research,1994,22:4673-4680.
[12] KUMAR S,TAMURA K,JAKOBSEN IB,et al.MEGA2:molecular evolutionary genetics analysis software[J].Bioinformatics,2001,17:1244-1245.
[13] SAITOU N,NEI M.The neighbor-joining method:a new method for reconstructing phylogenetic trees[J].Molecular Biology and Evolution,1987,4:406-425.
[14] APWEILER R,ATTWOOD TK,BAIROCH A,et al.InterPro-an integrated documentation resource for protein families,domains and functional sites[J].Bioinformatics,2000,16:1145-1150.
[15] COULSON RM,OUZOUNIS CA.The phylogenetic diversity of eukaryotic transcription[J].Nucleic Acids Research,2003,31:653-660.
[16] OHM RA,DE JONG JF,DE BEKKER C,et al.Transcription factor genes of Schizophyllum commune involved in regulation of mushroom formation[J].Molecular Microbiology,2011,81:1433-1445.

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备注/Memo

第一作者简介：孟丽(1984-)，女，博士，讲师，现主要从事食用菌遗传育种等研究工作。E-mail：mengli0121@126.com.

责任作者：李玉（1944-），男，博士，教授，现主要从事大型真菌分类，菌物资源保育，食用菌遗传与育种等研究工作。E-mail：yuli966@126.com.
谢宝贵（1962-），男，博士，教授，现主要从事食用菌遗传育种等研究工作。E-mail：mrcfafu@163.com.
基金项目：山东省自然科学基金（ZR2016CL13）。
收稿日期：2017―09―30

更新日期/Last Update: 2018-03-07