SHI Cong,LIU Dahao,SHEN Suwan,et al.Effects of Exogenous Melatonin Treatment on Quality and Reactive Oxygen Species Metabolism in Fresh-Cut Lettuce[J].Northern Horticulture,2023,(19):84-91.[doi:10.11937/bfyy.20225295]
外源褪黑素处理对鲜切莴苣品质与活性氧代谢的影响
- Title:
- Effects of Exogenous Melatonin Treatment on Quality and Reactive Oxygen Species Metabolism in Fresh-Cut Lettuce
- 文章编号:
- 1001-0009(2023)19-0084-08
- Keywords:
- melatonin; lettuce; fresh-cut; quality; active oxygen metabolism
- 分类号:
- TS 255.3
- 文献标志码:
- A
- 摘要:
- 以鲜切莴苣为试材，采用50、100、200 μmol·L-1褪黑素对鲜切莴苣浸渍处理5 min，置于4 ℃贮藏10 d，研究了褪黑素处理对鲜切莴苣贮藏品质和活性氧代谢的影响，以期为鲜切莴苣贮藏保鲜提供参考依据。结果表明：100 μmol·L-1褪黑素处理可有效延缓鲜切莴苣在贮藏期间的色泽、硬度、失重率和细胞膜透性的变化，抑制总酚含量的下降。此外，褪黑素处理提高了超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性及DPPH自由基清除能力，降低了鲜切莴苣超氧阴离子的生成速率，减少了过氧化氢和丙二醛的积累。因此，褪黑素处理可以提高鲜切莴苣的抗氧化能力，减少活性氧对自身的伤害，进而有效维持鲜切莴苣贮藏品质。
- Abstract:
- Taking fresh-cut lettuce as test material，fresh-cut lettuce were soaked with 50,100 μmol·L-1 and 200 μmol·L-1 melatonin solution for 5 minutes and stored at 4 ℃ for 10 days.The effects of melatonin treatments on quality and reactive oxygen metabolism of fresh-cut lettuce were investigated,in order to provide reference for the theoretical research on the storage and presercation of fresh-cut lettuce.The results showed that 100 μmol·L-1 melatonin treatment effectively delayed the changes of color,firmness，weight loss ratio and cell membrane permeability of fresh-cut lettuce during storage,inhibited the decrease of total phenols.Furthermore，the activities of SOD,POD,CAT and DPPH free radical scavenging activity in fresh-cut lettuce treated with melatonin were increased,O2 generation rate，the malondialdehyde and H2O2 content were decreased.Therefore,melatonin treatment improved the antioxidant capacity to reduce the damage of active oxygen to the fresh-cut lettuce,which further maintained effectively the storage quality of fresh-cut lettuce.
参考文献/References:
［1］杨晓哲,胡文忠,姜爱丽,等.鲜切莴苣生理生化变化及其保鲜技术的研究进展［J］.食品与发酵工业,2018,44(2):278-283.［2］李静,季悦,李美琳,等.切割方式对鲜切莴苣品质及抗氧化活性的影响［J］.食品科学,2018,39(3):268-273.［3］祝竞芳,汤静,游万里,等.氯化钙处理对鲜切莴苣品质及γ-氨基丁酸代谢的影响［J］.食品科学,2022,43(7):174-181.［4］MOON K M,LEE B,CHO W K,et al.Swertiajaponin as an anti-browning and antioxidant flavonoid［J］.Food Chemistry，2018，252：207-214.［5］刘云芬,廖玲燕,段振华,等.不同处理对鲜切莴苣褐变及贮藏品质的影响［J］.保鲜与加工,2020,20(4):15-20.［6］LI L，YI P，LI C，et al.Influence of polysaccharide-based edible coatings on enzymatic browning and oxidative senescence of fresh-cut lettuce［J］.Food Science & Nutrition，2021，9(2)：888-899.［7］PENG H，KANDEL J S，MICHELMORE R W,et al.Identification of factors affecting the deterioration rate of fresh-cut lettuce in modified atmosphere packaging［J］.Food and Bioprocess Technology，2020，13(11)：1997-2011.［8］XYLIA P,CHRYSARGYRIS A,TZORTZAKIS N.The combined and single effect of marjoram essential oil，ascorbic acid，and chitosan on fresh-cut lettuce preservation［J］.Foods，2021，10(3)：575.［9］FAN K,ZHANG M,BHANDARI B,et al.A combination treatment of ultrasound and ε-polylysine to improve microorganisms and storage quality of fresh-cut lettuce［J］.LWT，2019，113：108315.［10］PACE B，CAPOTORTO I，PALUMBO M et al.Combined effect of dipping in oxalic or in citric acid and low O2 modified atmosphere，to preserve the quality of fresh-cut lettuce during storage［J］.Foods，2020，9(8)：988.［11］RUSSEI J R，TAN D X，ZHOU Z,et al.Phytomelatonin：Assisting plants to survive and thrive［J］.Molecules，2015，20(4):7396-7437.［12］何欢,刘昭雪,张亚琳,等.外源褪黑素通过调控活性氧代谢减轻采后杏果实冷害［J］.食品科学,2022,43(5):168-174.［13］胡容平,范中菡,董义霞等.褪黑素对葡萄果实品质的影响［J］.北方园艺,2022(4):39-44.［14］WANG S Y，SHI X C，WANG R，et al.Melatonin in fruit production and postharvest preservation：A review［J］.Food Chemistry，2020,320：126642.［15］AGHDAM M S，FARD J R.Melatonin treatment attenuates postharvest decay and maintains nutritional quality of strawberry fruits (Fragaria × anannasa cv.Selva) by Enhancing GABA shunt activity［J］.Food Chemisty,2017，221:1650-1657.［16］朱玲玲,胡花丽,罗淑芬,等.褪黑素调控呼吸代谢及抗氧化活性延缓采后青花菜衰老［J］.农业工程学报,2018,34(3):300-308.［17］HU W，YANG H，TIE W，et al.Natural variation in banana varieties highlights the role of melatonin in postharvest ripening and quality［J］.Journal of Agricultural & Food Chemistry，2017，65(46)：9987-9994.［18］ZHAI R，LIU J，LIU F，et al.Melatonin limited ethylene production，softening and reduced physiology disorder in pear (Pyrus communis L.) fruit during senescence［J］.Postharvest Biology & Technology，2018，139(5)：38-46.［19］王海丹,普红梅,付坚,等.切分方式对鲜切黄瓜贮藏品质与微生物的影响［J］.北方园艺,2022(10):82-88.［20］WANG D，CHEN Q，CHEN W，et al.Melatonin treatment maintains quality and delays lignification in loquat fruit during cold storage［J］.Scientia Horticulturae，2021，284：110126.［21］WU Z，TU M，YANG X，et al.Effect of cutting on the reactive oxygen species accumulation and energy change in postharvest melon fruit during storage［J］.Scientia Horticulturae，2019，257：108752.［22］曹健康,姜微波,赵玉梅.果蔬采后生理生化试验指导［M］.北京：中国轻工业出版社，2017.［23］YI F，WANG J，XIANG Y，et al.Physiological and quality changes in fresh-cut mango fruit as influenced by cold plasma［J］.Postharvest Biology and Technology，2022，194：112105.［24］雷长毅,汪开拓,谭美琳,等.褪黑素处理对采后李果实Priming抗病性的诱导作用［J］.食品工业科技,2022,43(13):329-335.［25］谢晓宇,张飞,石洁,等.预冷处理结合低温贮藏对西兰花贮藏品质的影响［J］.食品工业科技,2021,42(7):302-310.［26］SHADMANI N，AHMAD S H，SAARI N，et al.Chilling injury incidence and antioxidant enzyme activities of Carica papaya L.Frangi′ as influenced by postharvest hot water treatment and storage temperature［J］.Postharvest Biology and Technology，2015，99：114-119.［27］TAN X L，FAN Z Q,KUANG J F et al.Melatonin delays leaf senescence of Chinese flowering cabbage by suppressing ABFs-mediated abscisic acid biosynthesis and chlorophyll degradation［J］.Journal of Pineal Research，2019，67(1)：e12570.［28］LUO H B，JIANG J，ZHANG L et al.Effect of gibberellic acid and 6-benzylaminopurine on lignification of fresh-cut zizania latifolia during refrigerated (1 ℃) storage［J］.Journal of Food Processing and Preservation，2012，37(5):864-869.［29］ZHANG Y Y，HUBER D J，HU M J，et al.Delay of postharvest browning in litchi fruit by melatonin via the enhancing of antioxidative processes and oxidation repair［J］.Journal of Agricultural & Food Chemistry，2018，66(28)：7475-7484.［30］黄鸿晖,顾里娟,李美琳,等.褪黑素处理对草莓品质与活性氧代谢的影响［J］.食品科学,2021,42(15):187-193.［31］徐利伟,岑啸,李林香,等.外源褪黑素对低温胁迫下桃果实蔗糖代谢的影响［J］.核农学报,2017,31(10):1963-1971.［32］刘帅民,胡康琦,刘港帅,等.外源褪黑素处理对鲜切芒果贮藏品质的影响［J］.食品科学,2020,41(21):160-166.
相似文献/References:
[1]张来军,贾敬芬.外源褪黑素对滇黄芩愈伤组织增殖和分化的影响[J].北方园艺,2013,37(08):106.
ZHANG Lai-jun,JIA Jing-fen.Effect of Melatonin on the Proliferation and Differentiation of Calli of Scutellaria amoena[J].Northern Horticulture,2013,37(19):106.
[2]夏彦辉,李贺年,卢凤刚,等.不同施氮量对莴苣品质及产量的影响[J].北方园艺,2012,36(21):29.
XIA Yan-hui,LI He-nian,LU Feng-gang,et al.Effect of Different Nitrogen Application on Lettuce Quality and Yield[J].Northern Horticulture,2012,36(19):29.
[3]刘健晖,周东升,王芳宇,等.重金属对莴苣种子萌发和幼苗生长的抑制效应[J].北方园艺,2014,38(15):1.
LIU Jian-hui,ZHOU Dong-heng,WANG Fang-u,et al.Inhibition Effect of Heavy Metal on Seed Germination and Seedling Growth of Lettuce[J].Northern Horticulture,2014,38(19):1.
[4]王卉,宁慧霞,刘敏,等.莴苣原生质体的分离方法[J].北方园艺,2012,36(11):109.
WANGHui,NINGHui-xia,LIUMin,et al.StudyontheMethodofIsolationProtoplastsofLactucasativaL.[J].Northern Horticulture,2012,36(19):109.
[5]杨亚珍,陈玉子,董社琴,等.印度梨形孢对莴苣增产提质效果的研究[J].北方园艺,2015,39(01):164.[doi:10.11937/bfyy.201501044]
YANG Ya-zhen,CHEN Yu-zi,DONG She-qin,et al.Research on the Effect of P.indica on Lactuca’s Yield and Quality[J].Northern Horticulture,2015,39(19):164.[doi:10.11937/bfyy.201501044]
[6]郭 金 岭１,智 利 红 １,张 歌２.玉米秸秆基质对无土栽培莴苣生长的影响[J].北方园艺,2011,35(13):0.[doi:10.11937/bfyy.2011013010]
,Ｅ ｆ ｆ ｅ ｃ ｔ ｏ ｆ Ｓ ｕ ｂ ｓ ｔ ｒ ａ ｔ ｅ ｏ ｆ Ｃ ｏ ｒ ｎ Ｓ ｔ ａ ｌ ｋ ｓ ｏ ｎ Ｇ ｒ ｏ ｗ ｔ ｈ ｏ ｆ Ｓ ｏ ｉ ｌ ｌ ｅ ｓ ｓ Ｃ ｕ ｌ ｔ ｕ ｒ ｅ ｏ ｆ Ｌ ｅ ｔ ｔ ｕ ｃ ｅ[J].Northern Horticulture,2011,35(19):0.[doi:10.11937/bfyy.2011013010]
[7]付 晓 忠,廉 华.钾素不同用量对莴苣硝酸盐积累的影响[J].北方园艺,2009,33(01):0.[doi:10.11937/bfyy.200901020]
FU Xiao -zhong,LIAN Hua.Effects of Different Amount Potassium Elements on the Nitrate Accumulation of Lettuce[J].Northern Horticulture,2009,33(19):0.[doi:10.11937/bfyy.200901020]
[8]张 玉 鑫,高 世 铭,王 晓 巍,等.河西走廊高海拔冷凉区茎用莴苣适宜密度与播期研究[J].北方园艺,2009,33(11):0.[doi:10.11937/bfyy.2009011023]
ZHANG Yu -xin,GAO Shi -ming,W ANG Xiao -wei,et al.Study on Planting Density and Sowing Date of AsparagusLettuce in Hexi Corridor High-altitude Cold Areas[J].Northern Horticulture,2009,33(19):0.[doi:10.11937/bfyy.2009011023]
[9]柳国强,谢爱方,林多,等.盐胁迫对叶用莴苣生长与品质的影响[J].北方园艺,2016,40(21):20.[doi:10.11937/bfyy.201621005]
LIU Guoqiang,XIE Aifang,LIN Duo,et al.Effects of Salt Stress on Growth and Quality of Lettuce[J].Northern Horticulture,2016,40(19):20.[doi:10.11937/bfyy.201621005]
[10]赵小红,罗庆熙,饶玲.外源褪黑素在低温胁迫下对黄瓜幼苗抗冷性的影响[J].北方园艺,2017,41(14):55.[doi:10.11937/bfyy.20164959]
ZHAO Xiaohong,LUO Qingxi,RAO Ling.Effects of Exogenous Melatonin on Chilling Tolerance of Cucumber Seedlings Under Low Temperature Stress[J].Northern Horticulture,2017,41(19):55.[doi:10.11937/bfyy.20164959]
备注/Memo
第一作者简介：师聪(1990-)，男，硕士，实验师，现主要从事农产品加工与贮藏等研究工作。E-mail：shicong@xzit.edu.cn.基金项目：国家自然科学基金青年资助项目（32102121）；江苏省自然科学基金青年资助项目(BK20210078)；徐州市重点研发计划（社会发展）资助项目（KC21273）。收稿日期：2022-12-30