LYU Ruiling,CHEN Shi,WANG Jie,et al.Genetic Diversity Analysis of Morchella Species in China Based on ISSR Marker[J].Northern Horticulture,2023,(08):107-114.[doi:10.11937/bfyy.20223042]
基于ISSR标记的我国羊肚菌栽培品种遗传多样性分析
- Title:
- Genetic Diversity Analysis of Morchella Species in China Based on ISSR Marker
- Keywords:
- Morchella; ISSR; clustering analysis; polymorphism
- 文献标志码:
- A
- 摘要:
- 以来源于我国12个省份49个羊肚菌样品为试验材料，采用ISSR-PCR分子标记法，研究我国羊肚菌不同地区栽培品种间的遗传关系和多样性，以期明确我国现有羊肚菌栽培品种之间的遗传关系，为羊肚菌核心种质资源开发及遗传育种提供参考依据。结果表明：最优的ISSR-PCR反应体系为10×PCR Buffer（Mg2-）为2.5 μL，Mg2+为20 mmol?L-1，DNA为1 μg，dNTP Mix为1 μL，Tap酶为0.4 μL，引物为1 μL，ddH2O补至25 μL。运用筛选出的5条ISSR引物进行羊肚菌的多态性分析，共获得111个电泳条带，其中110个为多态性条带，多态比率为99.10%。采用UPGMA法进行聚类分析，在相异系数为0.72时，可以将49个样品划分为3个大的类群。综上，我国羊肚菌栽培品种之间有较为丰富的遗传多样性。
- Abstract:
- The inter-simple sequence repeat (ISSR) molecular markers were used to explore the genetic diversity and genetic relationship of natural populations of 49 Morchella samples from 12 province regions．The aim of this study was to reveal the genetic diversity of Morchella resources，and analyze their genetic differences，so as to provide reference for the selection and breeding of Morchella resources．The results showed that the optimal ISSR-PCR reaction system was as follows 10×PCR Buffer (Mg2-) was 2.5 μL,Mg2+ was 20 mmol?L-1,DNA was 1 μg,dNTP Mix was 1 μL,Taq enzyme was 0.4 μL,primer was 1 μL,ddH2O supplemented to 25 μL.and a total of 111 bands were amplified with 5 primers，including 110 polymorphic bands (PPB),The proportion of PPB was 99.10%.The UPGMA method was used for cluster analysis,and 49 samples could be divided into three large groups when the number of different lines was 0.72.In conclusion,there is abundant genetic diversity among cultivated varieties of Morchella in China.
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备注/Memo
第一作者简介：吕锐玲（1982-）,女，博士，高级农艺师，现主要从事珍稀食用菌种质资源利用等研究工作。Ｅ-ｍail：lvruiling@hgnu.edu.cn．责任作者：郑永良（1969-），男，博士，教授，博士生导师，现主要从事农林生物质资源利用与转化等研究工作。Ｅ-mail：zhengyongliang＠163.com.基金项目：湖北省重点研发计划资助项目（2020BBB091）；经济林木种质改良与资源综合利用湖北省重点实验室开放课题资助项目（2021ZD09）；黄冈师范学院博士科研启动基金资助项目（2042020014）。收稿日期：2022-07-24