GONG Zuyun,HU Zhihui,WANG Yanjie.Effects of Exogenous EBR on Photosynthetic Physiology of Cowpea Under Waterlogging,Drought and Salt Stress[J].Northern Horticulture,2022,(23):9-20.[doi:10.11937/bfyy.20221484]
外源EBR对涝渍干旱和盐胁迫下豇豆光合生理的影响
- Title:
- Effects of Exogenous EBR on Photosynthetic Physiology of Cowpea Under Waterlogging,Drought and Salt Stress
- Keywords:
- brassinosteroids; waterlogging stress; drought stress; salt stress; cowpea; physiology and biochemistry
- 文献标志码:
- A
- 摘要:
- 以“鄂豇豆7号”和“江大紫豇1号”2个长豇豆品种为试验材料，采用对田间豇豆植株分别进行单盐胁迫、盐和干旱双重胁迫以及盐和涝渍双重胁迫，并喷施油菜素内酯（EBR）的方法，测定光合指标(净光合速率、胞间二氧化碳浓度、气孔导度和蒸腾速率)、抗氧化酶指标和丙二醛含量指标，研究了油菜素内酯对受外界胁迫的豇豆幼苗生理生化的影响，以期为外源油菜素内酯应用于缓解豇豆逆培提供参考依据。结果表明：在涝渍、干旱和盐胁迫条件下,豇豆叶片的气孔导度(Gs)、蒸腾速率(Tr)和胞间二氧化碳浓度(Ci)降低，导致气孔开合度降低，使光合作用的原料CO2不足而降低净光合速率(Pn)，外源EBR提高了涝渍、干旱和盐胁迫下豇豆叶片光合作用，外源 EBR 处理提高了豇豆的净光合速率,降低了胞间二氧化碳浓度、气孔导度、蒸腾速率。在涝渍、干旱和盐胁迫条件下,豇豆的超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）活性和丙二醛（MDA）含量均有所上升。
- Abstract:
- Taking ‘Ejiangdou 7’and ‘Jiangda Zijiang 1 Hao’two long cowpea varieties as test materials,the field cowpea plants were subjected to single salt stress,salt and drought dual stress,and salt and waterlogging dual stress respectively,the method of spraying brassinolide (EBR) was used,photosynthetic indicators (net photosynthetic rate,intercellular CO2 concentration,stomatal conductance and transpiration rate),antioxidant enzymes indicators and malondialdehyde content indicators were measured and detected,the effects of brassinolide on the physiology and biochemistry of cowpea seedlings under external stress were studied,in order to provide reference for the application of exogenous brassinolide to alleviate cowpea strress.The results showed that under the conditions of waterlogging,drought and salt stress,the stomatal conductance (Gs),transpiration rate (Tr) and intercellular CO2 concentration (Ci) of cowpea leaves decreased,resulting in the decrease of stomatal opening and closing degree,and the reduction of photosynthesis.Insufficient raw material CO2 reduced the net light rate (Pn),exogenous EBR improved the photosynthesis of cowpea leaves under waterlogging,drought and salt stress,and exogenous EBR treatment increased the net photosynthetic rate of cowpea,reduced the concentration of intercellular CO2,stomatal conductivity and transpiration rate.Under waterlogging,drought and salt stress conditions,the activities of SOD,POD,CAT,APX and MDA contet in cowpea all increased.
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备注/Memo
第一作者简介：龚祖运（1997-），男，硕士研究生，研究方向为遗传学。E-mail：770822650@qq.com.责任作者：胡志辉(1973-)，男，本科，研究员，硕士生导师，现主要从事植物生理生化遗传育种等研究工作。E-mail：1259769147@qq.com.基金项目：湖北省教育厅百校联百县-高校服务乡村振兴科技支撑行动计划资助项目（BXLBX0366）；湖北省豆类(蔬菜)植物工程技术研究中心开放基金资助项目（DLGC2021-03）；江汉大学2020级研究生科研创新基金资助项目（220710014）；湖北省科技厅2022年乡村振兴科技支撑资助项目（XCZXKJ2022000018）。收稿日期：2022-04-13