CHEN Bao-yin,WANG Cai-hong,CHU Qing-gang,et al.Effect of Exogenous Brassinolide on Growth and Anatomical Characteristics for Stems and Leaves of Dwarf Pear in vitro [J].Northern Horticulture,2014,38(11):7-11.
外源油菜素内酯对矮生梨试管苗茎、叶生长及解剖特性的影响
- Title:
- Effect of Exogenous Brassinolide on Growth and Anatomical Characteristics for Stems and Leaves of Dwarf Pear in vitro
- 文章编号:
- 1001-0009(2014)11-0007-05
- 分类号:
- S 661.2
- 文献标志码:
- A
- 摘要:
- 以矮生型梨试管苗为试材，通过在培养基中添加不同浓度外源油菜素内酯（BR）（0.001、0.010、0.100、1.000 mg/L），以不添加BR为对照，观察其对矮生梨试管苗生长的表型效应，以探讨矮生梨的矮生特性与外源BR的关系。结果表明：较高浓度的外源BR可明显促进矮生梨茎的高度和其节间长度的增加；另外，用石蜡切片法观察发现，1.000 mg/L BR处理可明显促进矮生梨试管苗茎和叶脉维管束的分化，提高叶肉细胞的密度和角质层的厚度。
- Abstract:
- Taking dwarfing pear of tube seedling as material,different concentrations of exogenous brassinolides (BR)（0.001,0.010,0.100,1.000 mg/L）were added to the media and the effect on phenotypes of the plantlets in vitro were observed,with no added BR as control,in order to reveal the relationship between the dwarf trait of pear and BR.The results showed that the higher concentration of BR increased the height and internode length of the plantlet.Furthermore,by the technique of paraffin section,it was observed that the treatment of 1.000 mg/L BR distinctively accelerated the differentiation of vascular bundles in stems and primary veins of leaves,and increased the density of mesophyll cells and the thickness of cuticle.
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备注/Memo
第一作者简介：陈宝印（1987-），男，山东临沂人，硕士研究生，研究方向为果树分子育种。E-mail：ba_oyin_love@126.com.
责任作者：王彩虹（1968-），女，陕西礼泉人，博士，教授，硕士生导师，现主要从事果树生物技术及遗传育种等研究工作。E-mail：chwang@qau.edu.cn.
基金项目：山东省科技发展计划资助项目（2011GNC11018）；
山东省良种工程-资源创新与利用资助项目（2011-2014）。
收稿日期：2014-03-13