[1]刘晓勤,张锐利.新疆红枣黑斑病根际土壤真菌群落组成及多样性的分析[J].北方园艺,2020,44(14):94-102.[doi:10.11937/bfyy.20194912]
　LIU Xiaoqin,ZHANG Ruili.Analysis on Composition and Diversity of Fungal Community in Rhizosphere Soil of Jujube Black Spot Disease in Xinjiang[J].Northern Horticulture,2020,44(14):94-102.[doi:10.11937/bfyy.20194912]
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新疆红枣黑斑病根际土壤真菌群落组成及多样性的分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年14 页码: 94-102 栏目: 出版日期: 2020-07-30
Title:
Analysis on Composition and Diversity of Fungal Community in Rhizosphere Soil of Jujube Black Spot Disease in Xinjiang
作者:
刘晓勤; 张锐利
（塔里木大学 生命科学学院，新疆生产建设兵团塔里木盆地生物资源保护与利用重点实验室，新疆 阿拉尔 843300）
Author(s):
LIU Xiaoqin; ZHANG Ruili
(College of Life Science,Tarim University,Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps,Alar，Xinjiang 843300)
关键词:
红枣; 根际土壤; 黑斑病; 真菌群落组成; 多样性
Keywords:
jujube; rhizosphere soil; black spot disease; fungal community composition; diversity
DOI:
10.11937/bfyy.20194912
文献标志码:
A
摘要:
以灰枣和骏枣根际土壤为试材，采用Illumina Miseq测序技术分析了不同地区不同品种红枣黑斑病病株及健株根际土壤真菌群落组成和多样性；对土壤理化性质与根际土壤真菌丰富度前20位优势菌属之间的相关性进行分析。通过探究南疆地区发生红枣黑斑病根际土壤真菌群落的多样性，以期揭示新疆红枣发病与其根际土壤真菌群落之间的相关性，为生物防治提供思路和方法。结果表明：阿克苏和喀什地区灰枣和骏枣的根际土壤真菌测序中共获得6 221 336个有效标签，17 239个OTUs共鉴定出21个门42个纲299个属，其中80%为子囊菌门和担子菌门。阿克苏地区灰枣根际土样和骏枣根际土样中的菌属分别聚类在一起，喀什地区灰枣根际土样和骏枣根际土样中的菌属分别聚类在一起。RDA分析表明，发病灰枣土样中有效磷和pH对土壤微生物群落结构的影响最大，发病骏枣土样中有机碳、速效钾和pH对土壤微生物群落结构的影响最大。红枣黑斑病根际土样中真菌群落组成丰富，同一地区不同红枣品种根际土真菌群落结构存在显著差异；发病红枣根际土壤真菌群落多样性与土壤环境因子之间存在一定相关性。
Abstract:
Rhizosphere soil of gray jujube and Junzao were taken as materials.The Illumina Miseq high-throughput sequencing technology was used to analyze the composition，abundance and diversity of rhizosphere soil fungi community in different varieties of jujube black spot disease and healthy plants in different regions.The correlation between soil physical and chemical properties and the top 20 dominant species in the rhizosphere soil fungi richness was analyzed.In order to explore the diversity of fungal community in rhizosphere soil of black spot disease in southern Xinjiang，the intrinsic relationship between Xinjiang jujube disease and soil fungal community was revealed from the perspective of fungal community of rhizosphere soil in diseased jujube，to provide ideas for biological control.The results showed that 6 121 336 effective tags were obtained from the fungal sequencing of rhizosphere soil samples of gray jujube and Junzao in Aksu and Kashi regions，and 17 239 OTUs identified a total of 21 phyla and 299 genera in 42 phyla，80% of which were ascus Mycophyta and Basidiomycota.The fungi in the gray jujube rhizosphere soil samples and Junzao rhizosphere soil samples in the Aksu area were clustered separately，and the gray jujube rhizosphere soil samples in the Kashi area and Junzao rhizosphere soil samples were clustered together.RDA analysis showed that available phosphorus and pH in the soil samples of the diseased gray jujube had the greatest effect on the soil microbial community structure，and organic carbon，available potassium and pH in the soil samples of the diseased Junzao had the greatest effect on the soil microbial community structure.The fungal community composition was rich in the rhizosphere soil samples of the jujube black spot.The rhizosphere soil fungi community structure of different varieties in the same area was significantly different.There was a certain correlation between the diversity of the rhizosphere soil fungal community and the soil environmental factors in the same region.

参考文献/References:

[1]付威,何荣,坎杂,等.红枣力学特性的试验研究[J].石河子大学学报(自然科学版),2013,31(4):518-522.[2]张栋海,李克福,赵思峰.新疆南疆矮化密植枣园三种红枣病害发生规律及其影响因素研究[J].北方园艺,2015(3):105-108.[3]向征,钟聪慧,胡军,等.新疆枣果黑斑病病原鉴定[J].新疆农业科学,2013，50(5):845-850.[4]蔡龙.红枣黑斑病发病研究及药剂防治试验[J].山西果树，2013(3):5-7.[5]TAN Y,CUI Y S,LI H Y,et al.Rhizospheric soil and root endogenous fungal diversity and composition in response to continuous Panax notoginseng cropping practices[J].Microbiol Res，2017，194(9)：10-19.[6]BAI L，CUI J Q，JIE W G，et al.Analysis of the community compositions of rhizosphere fungi in soybeans continuous cropping fields[J].Microbiol Res，2015，180:49-56.[7]TEIXEIRA L C R S，PEIXOTO R S，CURY J C，et al.Bacterial diversity in rhizosphere soil from Antarctic vascular plants of Admiralty Bay，maritime Antarctica[J].The ISME Journal，2010，4(8)：989-1001.[8]CHO J C，GIOVANNONI S J.Cultivation and growth characteristics of a diverse group of oligotrophic marine Gammaproteobacteria[J].Applied and Environmental Microbiology，2004，70(1)：432-440.[9]陈存红，王兰.阿拉尔垦区红枣黑斑病影响因素调查及绿色防控建议[J].新疆农垦科技，2017(6):33-35.[10]鲍士旦.土壤农化分析[M].北京：中国农业出版社，2000.[11]符泽，刘心，钟聪慧.阿拉尔垦区红枣黑斑病的发生规律及综合防治[J].新疆农垦科技，2015(7):33-35.[12]LIU J，WU F Z，YANG Y.Effects of cinnamic acid on bacterial community diversity in rhizosphere soil of cucumber seedlings under salt stress[J].Agr Sci China，2010，9(2)：266-274.[13]ZHANG D，MENG K X，HAO Y H，et al.Comparative proteomic analysis of cucumber roots infected by Fusarium oxysporum f.sp.cucumerium Owen[J].Physiol Mol Plant P，2016，96：77-84.[14]祖蕾,张晓婷,申强,等.不同土壤条件对越橘生长发育及果实品质的影响[J].北方园艺，2019(16):17-22.[15]KENNEDY A C，SMITH K L.Soil microbial diversity and the sustainability of agricultural soils[J].Plant Soil，1995，170(1)：75-86.[16]胡珍珠,潘存德,陈虹.新疆若羌县红枣生产园土壤肥力研究[J].河北农业大学学报，2019(3):76-80.[17]GAJERA H，DOMADIYA R，PATEL S，et al.Molecular mechanism of Trichoderma as bio-control agents against phytopathogen system-a review[J].Curr Res Microbiol Biotechnol，2013，1(3)：133-142.[18]JIN T,WANG Y,HUANG Y Y,et al.Taxonomic structure and functional association of foxtail millet root microbiome[J].GigaScience,2017,6(10):1-12.[19]DELGSDO-BAQUERIZO M,OLIVERO A M,BREWER T E,et al.A global atlas of the dominant bacteria found in soil[J].Science,2018,359(6373):320-325.[20]史功赋,赵小庆,方静,等.土壤微生物在植物生长发育中的作用及应用前景[J].北方农业学报,2019,47(4):108-114.

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备注/Memo

第一作者简介：刘晓勤（1988-），女，硕士研究生，研究方向为食品安全以及食品微生物。E-mail:348200557@qq.com.责任作者：张锐利（1975-），男，硕士，副教授，硕士生导师，现主要从事新疆红枣链格孢毒素污染发生规律及防治等研究工作。E-mail:zrl_p@sina.com.基金项目：NSFC-新疆联合基金重点资助项目（U1703234）；北京化工大学和塔里木大学联合基金资助项目（TDBHLH201705）。收稿日期：2020-03-16

更新日期/Last Update: 2020-09-15