WANG Jiayue,MENG Qingxue,CAO Lei,et al.Bulbils Induction Study of Asian Lily ‘Fangio’[J].Northern Horticulture,2020,44(24):62-69.[doi:10.11937/bfyy.20194322]
亚洲百合“红芯”珠芽诱导研究
- Title:
- Bulbils Induction Study of Asian Lily ‘Fangio’
- Keywords:
- lily; bulbil; in vitro induction; tissue culture; remove the top
- 文献标志码:
- A
- 摘要:
- 以“红芯”百合的嫩茎为外植体，采用组织培养的方法，研究不同消毒剂浓度及消毒时间、不同激素浓度配比对“红芯”离体诱导产生珠芽的影响；以“红芯”为试材，在试验基地的大棚内，对露地生长的植株进行去顶与喷施多效唑或矮壮素相结合的处理，研究植株上的珠芽诱导情况，以期通过这2类方式，为提高“红芯”的珠芽繁殖效率提供参考依据。结果表明：最适消毒剂浓度及时间为1% NaClO浸泡10 min；“红芯”诱导珠芽的最适培养基为MS+0.5 mg?L-1 6-BA+0.3 mg?L-1 NAA+90 g?L-1蔗糖；同一植株不同部位的珠芽诱导率为上部>中部>下部；对不同发育阶段的上部茎段研究表明，营养生长期的植株最适宜离体诱导珠芽。对自然生长的“红芯”进行创伤性诱导试验，发现去顶处理的最佳时期为现蕾初期；最佳诱导方式为去顶的同时对植株喷施多效唑。
- Abstract:
- Using the tender stems of the ‘Fangio’ lily as explants,the method of tissue culture was used,the effects of different disinfectant concentrations,disinfection times,and different hormone concentration ratios on the in vitro induction of ‘Fangio’ bulbils were studied.Using ‘Fangio’ as the test materials,in the greenhouse of the test base,the topping of the plants grown in the open field was combined with the spraying of paclobutrazol or chlormequat,the induction of bulbils on the plants were studied,in order to provide a reference for improving the efficiency of ‘Fangio’ bulbils by the two methods.The results showed that the optimal disinfectant concentration and time were immersed in 1% NaClO for 10 minutes;the optimum medium for ‘Fangio’ to induce bulbils was MS+0.5 mg?L-1 6-BA+0.3 mg?L-1 NAA+90 g?L-1 sucrose.The induction rate of bulbils in different parts of the same plant was upper>middle>lower;studies on the upper stem segments at different developmental stages showed that the plants in the vegetative growth stage were the most suitable for in vitro induction of bulbils.The traumatic induction experiment was carried out on the ‘Fangio’ of natural growth.It was found that the best period for the topping treatment was the beginning of the bud;the best induction method was to spray the paclobutrazol on the plant at the same time as the topping.
参考文献/References:
[1]石亮,罗凤霞,牛立新,等.亚铁杂交百合红芯Fangio的染色体与其加倍方法研究[J].植物研究,2006,26(6):699-702.[2]周春华,尤超,陈凝华.百合组织培养研究进展[J].北方园艺,2013(14):193-195.[3]姜福星,黄远祥,王丽娜,等.探索植物珠芽的奥秘[J].分子植物育种,2017,15(1):346-352.[4]陈心启,许介眉,梁松筠,等.中国植物志(第14卷)[M].北京:科学出版社,1980.[5]郑日如.生长延缓剂对东方百合植株生长和鳞茎养分代谢的影响研究[D].杭州:浙江大学,2011.[6]YANG P P,XU L F,XU H,et al.Histological and transcriptomic analysis during bulbil formation in Lilium lancifolium[J].Frontiers in Plant Science,2017（8）:1-14.[7]KAMENETSKY R,OKUBO H.Ornamental geophytes:From basic science to sustainable production[M].Boca Raton:CRC Press,2012.[8]王亚,李雪珂,张启翔,等.‘西伯利亚’百合组培快繁体系的优化[J].中国观赏园艺研究进展,2012(8):251-255.[9]HAN B H.The formation and growth of bulblet sections with swollen basal plate in Lilium oriental hybrid ‘casa blanca’[J].Journal of the Korean Society for Horticultural Science,1999,40(6):747-750.[10]KAPOOR R,KUMAR S,KANWAR J.Bulblet production from node explant grown in vitro in hybrid lilies[J].International Journal of Plant Production,2009,3(4):1-6.[11]张慧,杜运鹏,张铭芳,等.赤霉素及其抑制剂处理对卷丹和珠芽生长的影响[J].分子植物育种,2019,17(7):2350-2355.[12]吴昀.基于离体模式体系的百合小鳞茎发生发育机制研究[D].杭州:浙江大学,2016.[13]孙莹莹,杜禹珊,罗睿,等.不同植物生长调节剂对半夏珠芽产量及发育的影响[J].贵州农业科学,2015,43(8):217-219.[14]韦莉萍,韦绍龙,苏宾,等.兰州百合鳞茎再生繁殖体系的建立[J].南方农业学报,2014,45(5):742-748.[15]NIIMI Y,ONOZAWA T.In vitro bulblet formation from leaf segments of lilies,especially Lilium rubellum Baker[J].Scientia Horticulturae,1979,11(4):379-389.[16]WANG Q,KOHLEN W.Auxin depletion from the leaf axil conditions competence for axillary meristem formation in arabidopsis and tomato[J].The Plant Cell,2014,26(5):2068-2079.[17]ABRAHAM J.Functionally different PIN proteins control auxin flux during bulbil development in Agave tequilana[J].Journal of Experimental Botany,2015,66(13):3893-3905.[18]蔡国红,杨泉,李洪波,等.蔗糖、多效唑、ABA、KT对淮山薯试管珠芽诱导的影响[J].热带作物学报,2010,31(9):1458-1463.[19]兰金旭,李武高,龚攀.珠芽期不同措施对兰州百合生长的影响[J].蔬菜试验研究,2018(3):8-11.
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备注/Memo
第一作者简介：王嘉悦（1995-），女，硕士研究生，研究方向为园林植物。E-mail：15846577425@163.com.责任作者：樊金萍（1972-），女，博士，教授，现主要从事园林植物遗传育种等研究工作。E-mail：jinpingfan@neau.edu.cn.基金项目：黑龙江省自然科学基金资助项目（C2017030）；国家科技部重点研发资助项目（2016YFC0500306-02）。收稿日期：2019-11-21