SU Jun,CHEN Lu,ZHANG Xiaodong,et al.Cloned and Bioinformatics Analysis of HY5 Gene From ‘Yunhongli No.1’[J].Northern Horticulture,2020,44(10):39-44.[doi:10.11937/bfyy.20193540]
“云红梨1号”HY5基因的克隆及生物信息学分析
- Title:
- Cloned and Bioinformatics Analysis of HY5 Gene From ‘Yunhongli No.1’
- Keywords:
- HY5; ‘Yunhongli No.1’; photoreceptor; sequence analysis
- 文献标志码:
- A
- 摘要:
- 以“云红梨1号”的外果皮为试材，采用PCR技术，克隆出“云红梨1号”HY5基因cDNA的序列，进行生物信息学分析，研究了HY5基因的长度、编码、分子量、蛋白结构，并对HY5基因编码蛋白同源性进行了比较，以期为进一步对“云红梨1号”HY5基因功能和依光型花青素合成的机理研究奠定基础。结果表明：“云红梨1号”HY5基因全长为495 bp，编码164个氨基酸，其分子量为17.90 kD，为亲水蛋白，具有bZIP结构域；与苹果HY5基因具有高度的同源性，编码蛋白相似性为93%，表明其具有相类似或相同的功能。
- Abstract:
- The exocarp of ‘Yunhongli No.1’ was used as the test material to clone the sequence of HY5 gene cDNA of ‘Yunhongli No.1’ by PCR technology.The length,coding,molecular weight and protein structure of HY5 gene were studied,and the homology of HY5 gene coding protein was compared,in order to lay a foundation for further study on the mechanism of HY5 gene′s function and light-dependent anthocyanin synthesis of ‘Yunhongli No.1’.The results showed that HY5 gene of Pyrus pyrifolia contained open reading frames 495 bp cDNA,that encode deduced protein with 164 amino acid residues including bZIP domain.The molecular weight was 17.90 kD,belonged to hydrophilic proteinand.While the 93% sequence identfied to Malus pumila Mill.HY5 gene showed both of them had similar function.It established the foundation for further study the function and mechanism of photogenic anthocyanin synthesis of ‘Yunhongli No.1’.
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相似文献/References:
[1]郭继平.油菜HY5基因的电子克隆及生物信息学分析[J].北方园艺,2013,37(23):116.
GUO Ji-ping.In silico Cloning and Bioinformatics Analysis on HY5 Gene of Brassica napus[J].Northern Horticulture,2013,37(10):116.
备注/Memo
第一作者简介：苏俊（1980-），男，白族，硕士，研究员，现主要从事梨育种、栽培生理及品质调控等研究工作。E-mail：57470058@qq.com.责任作者：舒群（1963-），女，本科，研究员，研究方向为果树育种及栽培技术。E-mail:1057139643@qq.com.基金项目：国家自然科学基金地区科学基金资助项目（31760562）；云南省科技计划资助项目（2017FB062）；云南省技术创新人才培养计划资助项目（2015HB101）；现代农业产业技术体系建设专项资金资助项目（CARS-28-20）。收稿日期：2019-12-04