WU Yaowen,YANG Canxin,LI Xinhang,et al.Effects of Mn Stress on Growth,Physiological and Biochemical Characteristics of Daucus carota Seedling[J].Northern Horticulture,2019,43(10):23-30.[doi:10.11937/bfyy.20183569]
锰胁迫对野胡萝卜(Daucus carota)幼苗生长及理化特性的影响
- Title:
- Effects of Mn Stress on Growth,Physiological and Biochemical Characteristics of Daucus carota Seedling
- Keywords:
- Mn; Daucus carota; stress; physiological and biochemical; seedling growth
- 文献标志码:
- A
- 摘要:
- 野胡萝卜(Daucus carota)具有生物量大、适应性强、株型美观等特点，是锰矿废弃地污染治理的重要备选植物。以野胡萝卜为试材，采用盆栽试验的方法，研究了不同锰浓度(0~2 000 mg?kg-1)条件下野胡萝卜幼苗的生长及理化指标的变化特征。结果表明：随着锰浓度的升高，野胡萝卜幼苗生长呈现出‘低促高抑’现象，100～500 mg?kg-1锰胁迫可有效促进植物幼苗生长；当锰浓度高于1 500 mg?kg-1时，幼苗的生长受到抑制，其株高、根长、地下部干质量均较对照降低。随着锰浓度的升高，野胡萝卜幼苗叶片叶绿素、可溶性糖、可溶性蛋白质、游离脯氨酸含量以及超氧化物歧化酶(SOD)和过氧化物酶(POD)活性均呈现先增加后下降的变化趋势，并在500 mg?kg-1锰胁迫下达到最大值；而过氧化氢酶(CAT)活性则表现为逐渐下降趋势，丙二醛(MDA)含量则表现为先下降后上升的趋势。研究表明，野胡萝卜可通过增加渗透调节物质浓度和增强抗氧化酶活性等方式应对锰胁迫，从而在较高浓度锰胁迫下维持植株正常生长，表现出较强的耐锰胁迫能力。
- Abstract:
- Daucus carota has the characteristics of high biomass,strong adaptability and beautiful plant type.It is an important candidate plant for Mn-phytoremediation.Taking D.carota as test materials and revealing the physiological and biochemical adaptive mechanism to Mn stress,seedling growth and physiological and biochemical characteristics were compared for seedlings of D.carota growing under different Mn concentrations (0-2 000 mg?kg-1) by potted test methods.The results showed that with the increasing Mn concentration,seedling growth showed the trend of first increasing and then decreasing;100-500 mg?kg-1 Mn stress promoted the growth of D.carota seedlings;when the Mn concentration was over 1 500 mg?kg-1,the value of plant height,root length and underground dry weight were all smaller than those of the control.With the increasing of Mn concentration,the contents of chlorophyll,soluble sugar,soluble protein,proline,and activities of superoxide dismutase and peroxidase all showed the trend of first increasing and then decreasing,and reached the maximum under 500 mg?kg-1 Mn stress.However,catalase activity showed a downward trend with the rising Mn concentration,and malondialdehyde content showed a trend of first decreasing and then increasing.The results of the study revealed that D.carota could respond to Mn stress by increasing the concentration of osmotic adjustment substances and enhancing the activity of antioxidant enzymes,so as to maintain normal growth of plants under high Mn concentration and illustrated strong Mn tolerance.
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备注/Memo
第一作者简介：吴耀文（1992-），男，硕士研究生，研究方向为环境植物学。E-mail：1005787131@qq.com. 责任作者：刘文胜（1975-），男，博士，教授，硕士生导师，现主要从事环境植物学等研究工作。E-mail：liuwsairr@163.com.基金项目：湖南省自然科学基金资助项目（2016JJ4120）。收稿日期：2019-01-15