LI Xinyue,ZHANG Daoyuan,LI Jin.A Quick Method for Acquiring Malus sieversii′s Tissue Culture Seedlings[J].Northern Horticulture,2018,42(14):31-37.[doi:10.11937/bfyy.20174617]
一种快速获得野苹果组培苗的方法
- Title:
- A Quick Method for Acquiring Malus sieversii′s Tissue Culture Seedlings
- Keywords:
- Malus sieversii; seeds; seed embryo; tissue culture; rapid propagation
- 文献标志码:
- A
- 摘要:
- 以新疆野苹果成熟胚为试材，采用组织培养的方法，研究了新疆野苹果增殖及生根的最优培养基。结果表明：最适的增殖培养基为MS+0.4 mg·L-1 6-BA+0.1 mg·L-1 NAA，最适的生根培养基为1/2MS+1.0 mg·L-1 NAA，组培苗利用水培练苗后移栽成活率为65%。该研究建立了以新疆野苹果成熟胚为外植体的快速繁殖体系，并比较了新疆野苹果在不同增殖、生根培养基中的生长情况，以期为新疆野苹果的研究和保护提供帮助，为其快速繁殖和进一步的遗传转化提供参考依据。
- Abstract:
- The seed embryo of Malus sieversii was used as experimental materials，through tissue culture method the optimum medium for proliferation and rooting of Malus sieversii were stuided.The results showed that the optimal proliferation medium was MS+0.4 mg·L-1 6-BA+0.1 mg·L-1 NAA.Rooting medium was 1/2 MS+1.0 mg L-1 NAA.The survival rate of seedlings would raise to 65% by hardening-seedling with hydroponic culture before transplant to soil.The research laid a foundation for obtaining plenty of health seedlings in short time，which greatly benefited for wild apple forest ecological reconstruction in Tianshan Mountains，since they were seriously destroyed in past 10 years.
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备注/Memo
第一作者简介：李心悦（1992-），女，硕士研究生，研究方向为资源植物学。E-mail:xinyue1010@foxmail.com.责任作者：张道远（1973-），女，博士，研究员，博士生导师，现主要从事植物逆境分子生物学等研究工作。E-mail:zhangdy@ms.xjb.ac.cn.基金项目：新疆维吾尔自治区重大专项资助项目（201330122-3）。收稿日期：2018-02-24