[1]郑艳玲.多歧苏铁对高温和聚乙二醇处理的生理响应[J].北方园艺,2017,41(17):104-109.[doi:10.11937/bfyy.20170760]
　ZHENG Yanling.Physiological Response of Cycas multipinnata to High Temperature and PEG Treatments[J].Northern Horticulture,2017,41(17):104-109.[doi:10.11937/bfyy.20170760]
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多歧苏铁对高温和聚乙二醇处理的生理响应

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年17 页码: 104-109 栏目: 出版日期: 2017-09-15
Title:
Physiological Response of Cycas multipinnata to High Temperature and PEG Treatments
作者:
郑艳玲
西南林业大学 国家林业局西南地区生物多样性保育重点实验室，云南 昆明 650224
Author(s):
ZHENG Yanling
Key Laboratory of Biodiversity Conservation in Southwest China，State Forestry Administration，Southwest Forestry University，Kunming，Yunnan 650224
关键词:
多歧苏铁; 高温; PEG处理
Keywords:
Cycas multipinnata; high temperature; PEG treatment
DOI:
10.11937/bfyy.20170760
文献标志码:
A
摘要:
以多歧苏铁离体叶片为试材，研究了高温（25、35、45、55 ℃）和聚乙二醇（PEG）处理（0、0.05、0.10、0.15、0.20 g?mL-1）对多歧苏铁生理指标的影响，以期了解多歧苏铁的抗逆机理及生态适应策略。结果表明：各温度处理间丙二醛含量无显著差异；45 ℃处理后的过氧化氢酶（CAT）活性显著高于其它温度处理；与25 ℃处理相比较，35、45、55 ℃处理后的过氧化物酶（POD）活性显著下降，超氧化物歧化酶（SOD）活性显著升高；45 ℃和55 ℃处理后可溶性糖含量显著升高，但各温度处理间脯氨酸含量无显著差异。各浓度PEG处理间，色素含量及组成、Fv/Fm、丙二醛和脯氨酸含量及抗氧化酶（CAT、POD、SOD）活性均无显著差异，但0.10 g?mL-1和0.15 g?mL-1 PEG处理后的可溶性糖含量较其它浓度PEG处理后的显著提高。综上所述，多歧苏铁对高温具有一定耐受性，其中，可溶性糖和抗氧化酶（CAT、SOD）在渗透调节和抗氧化胁迫中起了一定作用。同时，所试PEG浓度并未引起多歧苏铁明显生理变化，该种可能具有较强的耐旱性。
Abstract:
To understand the ecological adaptive mechanisms of Cycas multipinnata under stress conditions，leaves collected from adult trees were used to determine the effects of different temperatures (25，35，45，55 ℃) and different concentrations of PEG (0.00，0.05，0.10，0.15，0.20 g?mL-1) on physiological characteristics of C.multipinnata.The results showed that MDA content did not vary significantly among different temperatures；CAT activity of leaves subjected to 45 ℃ was significantly higher than that of leaves subjected to other temperatures;compared to those of leaves subjected to 25 ℃，POD activity decreased and SOD activity increased significantly for leaves that were treated at 35，45，55 ℃；the soluble sugar content of leaves treated at 45 ℃ and 55 ℃ increased significantly，but the proline content of leaves did not vary significantly among temperature treatments.The pigment content and composition，Fv/Fm，MDA content，proline content and antioxidant enzyme (CAT，POD and SOD) activities did not vary significantly among leaves treated at various concentrations of PEG，but the soluble sugar contents of leaves treated at 0.10，0.15 g?mL-1 of PEG were significantly higher than that of leaves treated at other concentrations of PEG.In conclusion，C.multipinnata possessed relatively high tolerance to heat，and soluble sugar and antioxidant enzymes such as CAT and SOD played roles in osmotic adjustment and resistence of oxidative stress under high temperatures.The physiological process of C.multipinnata was not significantly affected by the tested concentrations of PEG which might suggest the relatively high tolerance of the species to drought.

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备注/Memo

作者简介：郑艳玲（1981-），女，博士，讲师，现主要从事植物种质资源保育及利用的教学与科研等工作。E-mail：flashingzyl@163.com.

基金项目：国家自然科学基金资助项目（31560093）。
收稿日期：2017-04-11

更新日期/Last Update: 2017-09-29