[1]王芳,李丹,张信旺,等.一株链霉菌对猕猴桃溃疡病的防效[J].北方园艺,2017,41(07):139-142.[doi:10.11937/bfyy.201707029]
　WANG Fang,LI Dan,ZHANG Xinwang,et al.Control Efficacy of Streptomyces.sp SY-L12 on Pseudomonas syringae pv.actinidiae Causal Agent of Kiwifruit Bacterial Canker[J].Northern Horticulture,2017,41(07):139-142.[doi:10.11937/bfyy.201707029]
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一株链霉菌对猕猴桃溃疡病的防效

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年07 页码: 139-142 栏目: 出版日期: 2017-04-15
Title:
Control Efficacy of Streptomyces.sp SY-L12 on Pseudomonas syringae pv.actinidiae Causal Agent of Kiwifruit Bacterial Canker
作者:
王芳1; 李丹1; 张信旺2; 李继广1; 周繁1; 金锡萱2
（1.沈阳化工研究院有限公司，辽宁 沈阳 110021；2.四川省猕猴桃工程技术研究中心，四川 成都 611600）
Author(s):
WANG Fang1; LI Dan1; ZHANG Xinwang2; LI Jiguang1; ZHOU Fan1; JIN Xixuan2;  FAN Jinzhe1; WEI Tingting1; JIAO Jiao1; XIN Xuecheng1
(1.Shenyang Research Institute of Chemical Industry Co.Ltd.，Shenyang，Liaoning 110021;2.Sichuan Engineering and Technology Research Center of Kiwifruit,Chengdu,Sichuan 611600)
关键词:
链霉菌; 猕猴桃溃疡病; 抑菌活性; 生物防治
Keywords:
actinomycetes; kiwifruit bacterial canker; antimicrobial activity; bio-control
DOI:
10.11937/bfyy.201707029
文献标志码:
A
摘要:
以猕猴桃溃疡病菌为试菌，采用液体共培养法和含菌抑菌圈法,研究了链霉菌SY-L12对猕猴桃溃疡病菌的生长抑制作用，并测定链霉菌发酵液对猕猴桃溃疡病的盆栽防效。结果表明：链霉菌SY-L12在培养24 h后其发酵液原液对病原菌的生长抑制率达94.0%，在28 ℃、150 r?min-1培养8~9 d条件下最利于活性物质的生产，发酵产物对猕猴桃溃疡病的盆栽防效为85.4%，优于氢氧化铜对照药剂。表明链霉菌SY-L12能够有效抑制猕猴桃溃疡病菌生长，作为生物农药具有开发应用前景。
Abstract:
Pseudomonas syringae pv.actinidiaeas was used as test fungi，the inhibition effect of Streptomyces.sp SY-L12 on Pseudomonas syringae pv.actinidiae was assessed by using co-culture and inhibition-zone methods.Then the control effect was studied in potted trails.The results showed that Streptomyces.sp SY-L12 inhibited the growth of Pseudomonas syringae pv.actinidiae with the inhibitory rate 94.0%.The best efficacy was found when Streptomyces.sp SY-L12 was grown at 28 ℃ with shaking at 150 r?min-1 for 8-9 days,the efficacy of fermentation broth was 85.4% which was higher than the control in potted trails.Therefore Streptomyces.sp SY-L12 might be considered as a new option for developing new integrated control strategies to reduce the disease.

参考文献/References:

 

[1]GUSTAVO D L，MATTEO E，FRANCESCO M，et al.Isolation and partial characterization of bacteriophages infecting Pseudomonas syringae pv.actinidiae，causal agent of kiwifruit bacterial canker[J].Journal Basic Microbiol,2014,54:1-12.

[2]MARSILIO R，PAUL C，ANNA R T.Bacterial canker on kiwifruit in Italy：Anatomical changes in the wood and in the primary infection sites[J].Bacteriology,2012,102(9):827-840.

[3]LI L B,DAN W J,TAN F F,et al.Synthesis and antibacterial activities of yanglingmycin analogues[J].Chem Pharm Bull,2015,63:33-37.

[4]申哲,黄丽丽,涂璇,等.植物内生放线菌活性物质防治猕猴桃溃疡病[J].中国生物防治，2008,24(4):329-334.

[5]ALAN C,de ZOYSA G H,VIJAYALEKSHMI S.Antimicrobial peptides against Pseudomonas syringae pv.actinidiae and Erwinia amylovora：Chemical synthesis，secondary structure efficacy，and mechanistic investigations[J].Peptide Science,2013,102(1):88-96.

[6]MARCO S.Field efficacy of chitosan to control Pseudomonas syringae pv.actinidiae,the causal agent of kiwifruit bacterial canker[J].European Journal of Plant Pathology,2014,140(4):887-892.

[7]苏婷，王桂跃，谢关林.壳聚糖对青枯劳尔氏菌生长及其生物膜形成的影响[J].植物保护，2013,39(1):89-92.

[8]张继文,杨春平,姬志勤,等.苦皮藤内生真菌Hd3菌株抑菌活性成分分离及结构鉴定[J].农药学学报,2009,11(2):225-229.

[9]CELLINILA,FIORENTINIL L,BURIANIL G,et al.Elicitors of the salicylic acid pathway reduce incidence of bacterial canker of kiwifruit caused by Pseudomonas syringae pv.actinidae[J].Annals of Applied Biology,2014,165:441-453.

[10]赵娜,王海丽,魏少鹏,等.放线菌C-411发酵液抑菌活性及活性成分[J].植物保护学报，2013,40(1):73-77.

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备注/Memo

第一作者简介：王芳（1981-），女，辽宁沈阳人，博士，高级工程师，研究方向为功能微生物应用。E-mail：wangfang1@sinochem.com.责任作者：周繁（1982-），女，重庆人，博士，高级工程师，研究方向为作物营养。E-mail：zhoufan03@sinochem.com.

更新日期/Last Update: 2017-04-19