XU Bin,LIAO Huanqin,YANG Huixiao,et al.Genetic Diversity Analysis on Camellia azalea Superior Clones Based on ISSR Markers[J].Northern Horticulture,2017,41(07):127-131.[doi:10.11937/bfyy.201707026]
杜鹃红山茶优良无性系遗传多样性的ISSR分析
- Title:
- Genetic Diversity Analysis on Camellia azalea Superior Clones Based on ISSR Markers
- Keywords:
- Camellia azalea; clones; genetic diversity; ISSR
- 文献标志码:
- A
- 摘要:
- 以39个杜鹃红山茶优良无性系为试材，采用ISSR分子标记方法，研究杜鹃红山茶优良无性系遗传多样性。结果表明：杜鹃红山茶无性系遗传多样性较高，采用筛选的26个ISSR引物共扩增出211个位点，多态位点180个，多态位点百分率(PPB)为85.3%,有效等位基因数1.6,Nei′s基因多样性指数(H)为0.345 1,Shannon多态性信息指数(I)为0.510 6，相似性系数为0.488 2～0.815 2，平均为0.668 8。UPGMA聚类分析得出，在遗传距离为0.360时,所有供试材料可以分为两大类:第Ⅰ类群是由4号和5号无性系组成,其它无性系构成第Ⅱ类群；当遗传距离为0.304时,又可将第Ⅱ类群分为6组。
- Abstract:
- Camellia azalea superior clones were used as test materials,the genetic diversity of Camellia azalea superior clones was evaluated by using ISSR markers.The results showed that the genetic diversity of C．azalea clones was abundant,a total of 211 bands were obtained in which 180 bands(85.3%)were polymorphic,the average effective number of alleles was 1.6,Nei′s gene diversity indices(H) was 0.345 1 and ShannonNei′s information index(I) was 0.510 6,the genetic similarity coefficients among the tested clones ranged was 0.488 2-0.815 2,with an average of 0.668 8.Cluster analysis was conducted by using UPGMA method,it was concluded that the 39 clones of C.azalea could be divided into 2 groups(the genetic distance was 0.360),and the second group included 6 subgroups (the genetic distance was 0.304).
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备注/Memo
第一作者简介：徐斌（1974-），男，博士，教授级高级工程师，现主要从事观赏植物研究与开发利用等研究工作。E-mail:xubin@sinogaf.cn.基金项目：广东省科技计划资助项目（2016A020206001）。