GAO Qiaoni,XIE Shuyu,ZHANG Yuejin,et al.Screening for Bagging Culture Conditions of Polyporus[J].Northern Horticulture,2017,41(05):130-133.[doi:10.11937/bfyy.201705031]
猪苓袋料培养条件筛选
- Title:
- Screening for Bagging Culture Conditions of Polyporus
- 文献标志码:
- A
- 摘要:
- 以猪苓为试材,采用PDA培养基及袋料培养真菌的传统方法,对猪苓在不同培养条件下菌丝的生长及菌核形成情况进行了分析,采用划线法测量猪苓菌丝生长速度,用观察计数法对猪苓菌核形成数量、大小、位置进行统计分析。结果表明:猪苓菌种袋料培养的最佳条件为25 ℃、24 h黑暗培养、料水比1∶1.4(kg?L-1);最适合猪苓菌种袋料培养的培养基配方为木屑50%、棉籽壳30%、麸皮18%、石膏1%、生石灰0.4%、蔗糖0.5%、KH2PO4 0.1%;明确了不同培养条件对猪苓菌丝生长发育及菌核形成的影响,为进一步开展猪苓种苓工厂化生产提供参考依据。
- Abstract:
- Taking Polyporus umbellatus as test material,the mycelia growth and sclerotia formation of Polyporus umbellatus in different culture conditions by the traditional method of culturing fungi with PDA medium and bag material were studied.The mycelia growth rate of Polyporus umbellatus was measured by the scribe method,and the number,size and position of sclerotia formation were analyzed by counting method.The results showed that the best condition for Polyporus substitute cultivation was:25 ℃,24 hours of darkness,the ratio of material to water of 1∶1.4(kg?L-1).The most suitable formula for bagging Polyporus cultivation was:sawdust 50%,the cotton seed hull 30%,bran 18%,gypsum 1%,quick lime 0.4%,sucrose 0.5%,KH2PO4 0.1%.The effects of different culture conditions on mycelia growth and sclerotia formation of Polyporus umbellatus were clarified,which could provide reference for the further production of Polyporus umbellatus
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备注/Memo
第一作者简介:高巧妮(1992-),女,硕士研究生,研究方向为中药材规范化生产。E-mail:576993578@qq.com.责任作者:张跃进(1960-),男,硕士,教授,研究方向为中药材GAP规范化栽培技术。E-mail:qxzhang@126.com.基金项目:陕西省科技统筹资助项目(2016KTCL02-10);濒危药材猪苓规范化种植与饮片标准化生产基地建设资助项目(国家工信部项目)。